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CILIATEMOLBIO-L  January 2008

CILIATEMOLBIO-L January 2008

Subject:

2008 Tetrahymena Forward Genetics Course

From:

Robert Coyne <[log in to unmask]>

Date:

Mon, 14 Jan 2008 18:18:18 -0500

Content-Type:

text/plain

Parts/Attachments:

Parts/Attachments

text/plain (40 lines)

Dear Colleagues,
 As many of you know, we are working on developing a set of resources to allow the cloning of
Tetrahymena genes by functional complementation of mutant phenotype; i.e. “cloning by
complementation”.  Results so far are promising that the method will prove successful.  We hope
that this resource will not only allow molecular analysis of existing classical genetic mutations, but
spur further use of Tetrahymena as a model organism for forward genetics.  However, many
researchers trained in molecular, cellular, and reverse genetics methods are unfamiliar with the
tools of Tetrahymena forward genetics.  Therefore, we plan to present a “crash course” in these
methods this summer, hosted by Ed Orias and Eileen Hamilton on the campus of the University of
California at Santa Barbara.  This course will take place July 14-19, 2008 (participants should plan
on arrival on the 13th, departure on the 20th; there is still a possibility we may have to finish by
the 18th, with departure on the 19th, depending on residence hall availability; we will know the
end date for sure by early February.) We have significant support from our NIH grant to cover
room and board at a UCSB facility, course materials, and a portion of travel expenses, depending
on the total number of attendees.  Currently, we anticipate limiting enrollment to about 15
attendees.
 The course will be fully hands-on and cover many genetic methods including rapid single-cell
isolation, performing crosses, mapping mutants to a chromosome arm by nullisomic matings, and
mating type determination. Participants will be encouraged to bring along an unmapped mutation
from their own freezers to map.  We will also cover methods for mass transformation that can be
used with the new cloning by complementation library (under development) and for identifying
the rescuing DNA fragment from that library.  In order to squeeze all these methods into a six-day
schedule, the course will be run in some ways like a televised cooking class (“We put ze cells in ze
incubator, and Voila!  Here are ze cultures as zey would appear two days later!”), although in the
end, every student will get to perform every step of each experiment.
 Our plans for this course were discussed at the FASEB Ciliate Molecular Biology Conference in
Tucson in July 2007.  At that time, a possible forward and reverse genetics combo course was
discussed, but since then, it has been decided to run only the forward genetics component in
2008.  We also collected names of researchers interested in attending the course.  At this time, we
need a recount!  The contract with UCSB to reserve the facilities must be signed within a short
time, so if you are seriously interested in attending this course (even if you already signed the
sheet in Tucson!), please let us know ASAP (by this Friday, January 18).  Your response does not
yet imply an iron-clad commitment to attend, but at least a quite serious interest. A definitive
commitment will be needed by June 13. If you have questions, please email us.
 We look forward to seeing you this summer in beautiful Santa Barbara!

Regards,
Bob Coyne ([log in to unmask])
Ed Orias ([log in to unmask])
Eileen Hamilton ([log in to unmask])

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