We would like to undertake some yeast 1- and 2-hybrid screens of
ciliate libraries. We imagine that to do this, we need a yeast
background that will suppress the ciliate glutamine codons that
yeast recognize as stop codons relatively effectively
UAG ->glutamine). There has been work done with E. coli suppressors
for ciliate gene expression, but we would like to have the entire
yeast genetic toolbox for the the hybrid screens.
Does anyone know of anything that might help us out in this endeavor?
Thanks so much,
Thomas G. Doak
Research Assoc. Domain Science Liaison
Department of Biology, Indiana University National Center for Genome Analysis Support
1001 East Third Street (NCGAS)
Bloomington, IN 47405