Hi All,

We would like to undertake some yeast 1- and 2-hybrid screens of ciliate libraries. We imagine that to do this, we need a yeast background that will suppress the ciliate glutamine codons that yeast recognize as stop codons relatively effectively (UAA and
UAG ->glutamine). There has been work done with E. coli suppressors for ciliate gene expression, but we would like to have the entire yeast genetic toolbox for the the hybrid screens.

Does anyone know of anything that might help us out in this endeavor?

Thanks so much,
Tom
--

Thomas G. Doak                                                                                                                

Research Assoc.                                                                                                                   Domain Science Liaison

Department of Biology, Indiana University                                                                National Center for Genome Analysis Support

1001 East Third Street                                                                                                       (NCGAS)

Bloomington, IN  47405                                                                                                    http://ncgas.org

812-856-0115

http://www.indiana.edu/~lynchlab/Thomas_Doak.htm 


--
Thomas G. Doak
Research Assoc. Domain Science Liaison
Department of Biology, Indiana University       National Center for Genome Analysis Support
1001 East Third Street  (NCGAS)
Bloomington, IN  47405
812-856-0115
http://www.indiana.edu/~lynchlab/Thomas_Doak.htm   http://ncgas.org