I'm happy to announce that the Tetrahymena Functional Genomics Database (TetraFGD) was updated, with the
RNA-Seq data at different stages integrated into the TetraFGD
Gbrowse. Tetrahymena thermophila RNA-Seq coverage and RPKM
(Reads Per Kilobase exon per Million mapped reads, relative expression level)
values at three stages (total five states) were showed in Gbrowse, inluding 1.
Growth (middle log phase, ~3.5X105 cells/ml ); 2.
Starvation 3 hours and 15 hours; 3. Conjugation 2 hours and 8 hours.
You can search the data
using Gene ID in the 2008 genome annotation at: http://tfgd.ihb.ac.cn/tool/seqmaps .
Another news, my lab
is working on sequencing the Mac genomes of 13 Tetrahymena
(T. amerricanis, T. australis, T. bergeri, T. corlissi, T. epidokyrea, T. hegewischi, T. paravorax, T. patula, T. pigmentosa, T. pyriformis, T. rostrata, T. shanghaiensis, T. vorax
). Now, all sequencing have been done, and seven of them
(T. bergeri, T. corlissi, T. empidokyrea, T. paravorax, T. pyriformis, T. shanghaiensis, T. vorax)
have been completed assembling and got good results and are annotated
one by one. I hope this information is helpful to
avoid unnecessary duplication among colleagues and welcome to the
collaboration with my lab.
Wei Miao, PhD
Laboratory of Protozoan Functional Genomics
Institute of Hydrobiology, Chinese Academy of Sciences