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Dear Erhan,
We often had a similar problem. While Normocin does not help, Normocure (from the same company) does. We use it 1:500 in small cultures; after 2-3 days, the cells are usually cured.
Best regards,
Josef Loidl
Von: Ciliate Molecular Biology [mailto:[log in to unmask]] Im Auftrag von erhan aslan
Gesendet: Donnerstag, 19. Juli 2018 17:47
An: [log in to unmask]
Betreff: Tetrahymena culture contamination
Dear Ciliate Community,
I am struggling with contamination in my Tetrahymena cultures for a while. Routinely used antibiotics (pen-strep, amphotericin) don't work. I used normocin from Invitrogen in conbination with pen-strep or kanamycin to get rid of them but no success again. Contaminated wells appear whitish and cloudy (Please see attachment). I noticed that this contamination generally occurs after biolistic bombardment. Sterile cabinet was tested for the possible contamination source but it seems OK. I extensively sterilize the inner chamber of the biolistic gun (which is in our sterile cabinet) and other components with alcohol prior to shot but they show up during passaging when I increase the pm concentration for example.
Many thanks in advance for your any kind of suggestions.
Best regards.
--
Erhan Aslan
Anadolu University
Graduate School of Sciences
Department of Molecular Biology
Eskisehir-Turkey
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