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Wed, 10 Oct 2001 15:00:42 -0400 |
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<000e01c151ae$c9dc4560$7e7675cb@b9n9a3> |
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Regarding the concentration of alcohol (ethanol) to use to preserve
molluscan tissue, I will put forward the following opinion:
For DNA studies - at least 95%.
For general storage - 80%.
Some people suggest 70-75% for general storage, howver, the solution will
draw water out of the tissue and become dilute. You should place the
tissue in a volume of ethanol about 10X the volume of the tissue and after
24-48 hours, replace the solution with fresh ethanol. By using 80% you
are giving yourself a safe margin of error against the replaced solution
becoming too dilute. It appears that autolosis begins at an alcoholic
concentration about 66%.
Regards,
Charlie
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Charlie Sturm, Jr
Research Associate - Section of Invertebrate Zoology
Carnegie Museum of Natural History, Pittsburgh, PA
Assistant Professor - Family Medicine
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