Dear everyone... A question regarding biolistic tranformation of Tetrahymena MIC/MACs: Does anyone have experience using the "small" gene gun: the Helios Gene Gun from BioRad (that actually looks like a gun)to biolistic transform Tetrahymena MIC and MAC nuclei ? E.i. can the Helios gun be used instead of the larger stationary PDS-1000/He System gene gun (BioRad) that is commonly used ? Hope you can help me. All the best, Kasper ----- Kasper L. Andersen [log in to unmask] / [log in to unmask] Postdoc Henrik Nielsen lab Dept. of Cellular and Molecular Medicine University of Copenhagen Denmark