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Created 05/09/2000 List_Affiliation UGA Department of Cellular Biology
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Wild-Type Paramecium tetraurelia?
Aufderheide, Karl J
Fri, 13 Jun 2025 21:24:27 +0000
Reply
I have just finished a year and a half medical adventure that ended with my own open heart surgery. Many of my lines of P. tetraurelia have been lost during my multiple hospital stays.

I really need wild-type paramecia, mating type O, to restore my back stock collection and allow me to start supervising undergraduate projects in mating inheritance genetics demonstrations and in mutation projects. I have mating type E still, but the mating type O line (stock 51s) died off during my multiple illnesses.

[More ...]
Reply
No Replies
Available PDS-1000|He System
Patrick Jiang
Mon, 19 May 2025 10:10:51 -0400
Reply
Dear Ciliate community members,

If you know anyone is interested in a full set of working biolistic
PDS-1000|He System (Biorad, 1652257), please have him/her contact me (
[log in to unmask]) by Tuesday (5/20) night.
The idea is only the cost of Fedex/UPS shipping from Arlington, MA, U.S.
and a signed donation/transfer letter are needed.

Best,
Patrick.
Reply
No Replies
Research at our university
Kapler, Geoffrey
Sat, 26 Apr 2025 01:47:21 +0000
Reply
Dear Members of the Ciliate Molecular Biology Community-
I was recently contacted by Anthony Carter- a retired NIH Program Director. He is seeking input on how faculty members, graduate programs and universities are being impacted by federal attacks on NIH and NSF-funded research budgets, university research infrastructure and DEI-related issues (including NSF Broader Impact statements). I gave him my overview of how the diverse ciliate research community, spanning R1 research universities and PUIs, has worked collaboratively over the years to advance science and train the next generation of researchers, and what is currently happening at Texas A&M.

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Reply
No Replies
Jean Kelly Nanney
Andrzej Kaczanowski
Tue, 22 Apr 2025 13:45:18 +0200
Reply
Dear friends , I have just received the very sad News that Jean Nanney, who
was widowed by David Nanney, passed away Yesterday,
Andrzej Kaczanowski
Reply
No Replies
Postdoc Positions in cryo-ET / cell biology
Daniela Nicastro
Sun, 30 Mar 2025 12:39:46 +0000
Reply
Dear All,

We have open Postdoctoral positions in Structural & Cell Biology in the Nicastro Lab at UT Southwestern in Dallas.

For details, please see:
https://www.utsouthwestern.edu/research/postdoctoral-scholars/assets/cell-structural-bio-nicastro.pdf

Please spread the word or contact me at [log in to unmask]<mailto:[log in to unmask]>. If you are applying, kindly include your CV.

Learn more about our lab here: www.utsouthwestern.edu/labs/nicastro/<http://www.utsouthwestern.edu/labs/nicastro/>

Best wishes,
Daniela
Reply
No Replies
Announcement of 2025 Ciliate Molecular Biology Conference
[log in to unmask]
Tue, 11 Feb 2025 10:48:01 +0800
Reply
Dear Colleagues,

I am pleased to announce that the 2025 Ciliate Molecular Biology Conference will be held in Wuhan, China, from July 25 to 28, 2025.

Meeting and registration information is available at https://cmb2025.casconf.cn/.

This conference provides an excellent opportunity for scientists to present their research, connect with colleagues, and learn about the latest advances in ciliate biology. Please encourage your students, postdocs, and peers to participate and share their work at this event.

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No Replies
Announcing the 2025 Midwest Protozoology Society Meeting
Mark Slabodnick
Wed, 29 Jan 2025 13:42:22 -0600
Reply
Dear colleagues,

I would like to announce that the 2025 Midwest Protozoology Society meeting
will be held on *Saturday April 26th at Knox College in Galesburg,
Illinois. *This year we will welcome Jim Forney from Purdue University as
our keynote speaker!

For those in the region, this is a great opportunity for undergraduate
students to present their work in a poster or talk, meet with colleagues,
and also hear about great research on ciliates and other protists.

[More ...]
Reply
No Replies
TetraFGD access?
Suzanne Lee
Mon, 27 Jan 2025 02:27:24 +0000
Reply
Dear Ciliate Community,

I recently tried to access the resources on TetraFGD and was surprised to find that I can’t get through (http://tfgd.ihb.ac.cn). Is this something others are experiencing or have insights into what might be preventing access?

With thanks in advance,
Suzanne

----
Suzanne R Lee, PhD
Associate Professor
Biology Department
Western Washington University
Reply
Show Replies 2 Replies
Re: TetraFGD access?
JUAN CARLOS GUTIERREZ FERNANDEZ
Mon, 27 Jan 2025 12:10:24 +0100
Dear Suzanne Lee,

Certainly the TetraFGD website does not work or cannot be accessed. I think
it was replaced by the Ciliate.org website (which does work!).
Another website that has not been working since last December (2024) is the
Ciliate Comparative Database (http://ciliates.ihb.ac.cn/tcgd/), and I don't
know why.
I don't know if this information can help you, and by the way it could
serve to find out the reason for the inaccessibility of the Ciliate
Comparative Database website (if anyone knows ? )

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Re: TetraFGD access?
Naomi Stover
Mon, 27 Jan 2025 10:09:13 -0600
Hi everyone,

I've noticed that TetraFGD is down as well. Ciliate.org has not replaced
this vital resource. We've simply stored emergency backup copies of the
microarray graphs to display to the community when they're doing site
maintenance or have other downtime. Aside from these, we don't hold any of
the expression values, browser data, or search tools that are unique to
their site. Our links to their site will operate again once TetraFGD
returns.

[More ...]
ASCB Ciliate Lunch 2024
Brian Bayless
Mon, 9 Dec 2024 11:44:18 -0500
Reply
Hi ASCB going ciliate community,

I hope everyone is doing well this year. The ASCB meeting is in San Diego and I would like to organize a gathering for all of our community that are attending. I am aiming for Tuesday the 17th around 12:30pm. If you, or any of your students/postdocs, would like to attend please fill out the following Google form or email me at [log in to unmask] no later than Tuesday the 10th of December so I can secure a lunch spot.

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No Replies
Humboldt Research Fellowship Programme
Luisa Jimenez
Tue, 15 Oct 2024 01:33:27 -0400
Reply
Dear All,
I have been informed that as member of a German university, I qualify to be host of researchers who want to come to do research in Germany under the Humboldt Research Fellowship Programme.

In my lab, I am interested in study ciliates and amoeba, as main predators of bacteria. With the help of collaborators in the TUM I am creating a lab 50% wet lab, 50% bioinformatics.

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No Replies
Related Flow Question
Wei-Jen Chang
Tue, 10 Sep 2024 11:38:31 -0400
Reply
Hello,

Kind of relating to what Luisa is asking. Has anyone tried using Flow
Cytometry to distinguish live (viable) and dead *Tetrahymena *cells? The
dyes we used did not do so well and we wonder if anyone has done this
before.

Thanks,

WJ
Reply
No Replies
Fixing solution for mating
Luisa
Tue, 10 Sep 2024 11:04:59 -0400
Reply
Dear Community,
We are trying to distinguish mating cells from non-mating using Flow cytometry signals.
Since we use a capillary system we want to fix the cells before, in case the shear forces will separate the cells.
We have been trying PFA in final concentration of 1.7% and it works but only for one person in the lab. He says that the speed in which the high concentrated PFA (25%) is added makes a big difference.
I need to find a way to fix the cells so that they do not separate and it is not operator dependent.
Do you

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Show Replies 1 Reply
Re: Fixing solution for mating
Jennifer Fricke Pinello
Tue, 10 Sep 2024 10:51:02 -0600
Hi Luisa & Wei-Jen,

We used an eBioscience fixation buffer
<https://www.thermofisher.com/order/catalog/product/00-8222-49> for
fixation of *Tetrahymena* mating pairs and single cells prior to flow
cytometry in the attached two papers.
I found this was a pretty gentle 2X fixative solution that preserves the
mating pairs well for either immunofluorescence or flow cytometry. For some
reason I always thought that this eBioscience buffer was essentially just a
~4% PFA solution, so alternatively, you could try that too as a 2X stock
solution and see if that works about the same? Perhaps diluting directly
into live cells with a 25% stock is too

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Full Time Teaching Professor Position: University of Colorado Boulder
Kristin Ann Moore
Mon, 19 Aug 2024 15:00:00 +0000
Reply
Dear Ciliate Community,

CU Boulder has an opening for a full-time teaching professor position in the Molecular, Cellular, and Developmental Biology Department starting spring 2025. This person will be responsible for leading a large-scale Course-based Undergraduate Research Experience (CURE) style course that focuses on cell and molecular biology using Tetrahymena as a model organism.

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Tenure Track Cell biology position at St. Olaf College
Kim Kandl
Fri, 2 Aug 2024 19:18:09 -0500
Reply
Dear Colleagues,

We are searching for a tenure-track Cell Biologist in the Biology
department here at St. Olaf College.

The *full job ad is posted here
<https://fa-ewur-saasfaprod1.fa.ocs.oraclecloud.com/hcmUI/CandidateExperience/en/sites/CX_3/job/922>
*and
there is a brief description below. Please share this link with anyone you
know who might be interested in this position. Thank you for your help
spreading the word!

Review of applications begins on Sept 3, 2024.

[More ...]
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No Replies
Assistant Professor of (Cell) Biology
Wei-Jen Chang
Wed, 24 Jul 2024 19:24:05 -0400
Reply
Hello,

My department at Hamilton College is searching for a tenure-track position
in cell biology due to a recent retirement. Details of the position and the
link to the application can be found at: https://apply.interfolio.com/150198

Please help spread the word, and I am happy to help answer any questions.

Best,

WJ
Reply
No Replies
Virtual Ciliate Molecular Biology Conference Schedule
Marcella Cervantes
Wed, 17 Jul 2024 21:19:41 -0500
Reply
Hello Everyone,
The schedule and abstracts for the virtual Ciliate Molecular Biology
meeting on July 23rd and 24th is attached.

Register in advance for this meeting:
https://us06web.zoom.us/meeting/register/tZItf-morTotE9R91s86NK3-_6GH-y-Skzn2

After registering, you will receive a confirmation email containing
information about joining the meeting.

The meeting opens thirty minutes before we begin each day.

We hope to see you then.
Reply
No Replies
A Call for Poster Abstracts!
Megan Valentine
Mon, 8 Jul 2024 10:48:58 -0400
Reply
Hello All!
Please send your poster abstracts for this summer's virtual ciliate research conference! The conference will be held July 23rd - 24th from 9 am to 12 pm EDT. This virtual conference will have a selection of invited speakers who will be contacted directly and posters for anyone who would like to present! Posters will be available for viewing overnight. Poster presenters can submit their abstracts in the following research focus areas: Motility (cilia, basal bodies, and flagella), Cell biology, Evolutionary biology, Gene expression, Developmental genomic rearrangement, and Developing undergraduate research. Prizes will be awarded to the best undergraduate

[More ...]
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No Replies
call for poster abstracts
Marcella Cervantes
Wed, 12 Jun 2024 11:27:41 -0400
Reply
Hello All!
Please begin sending your poster abstracts for this summer's virtual
ciliate research conference! The conference will be held July 23rd - 24th
from 9 am to 12 pm EDT. This virtual conference will have a selection of
invited speakers who will be contacted directly and posters for anyone who
would like to present! Posters will be available for viewing overnight.
Poster presenters can submit their abstracts in the following research
focus areas: Motility (cilia, basal bodies, and flagella), Cell biology,
Evolutionary biology, Gene expression, Developmental genomic rearrangement,
and Developing undergraduate research. Prizes will be awarded to the best


[More ...]
Reply
No Replies
Looking for a classical paper by Peter Bruns
Kazufumi MOCHIZUKI
Fri, 7 Jun 2024 14:08:54 +0000
Reply
Hi Ciliate Biology Community,

I am looking for the following classical paper written by Peter Bruns:
Bruns, P. J., Brussard, T. B., and Merriam, E. V. (1982). In vivo genetic engineering in Tetrahymena. Acta Protozool. 22, 31-44.

I found that the corresponding pages of Acta Protozoologica contain an unrelated (but still Tetrahymena!) paper. Moreover, Vol 22 of Acta Protozoologica was actually published in 1983. I also checked the previous volume published in 1982 but again I did not find the paper.

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Show Replies 1 Reply
Re: Looking for a classical paper by Peter Bruns
Kazufumi MOCHIZUKI
Fri, 7 Jun 2024 14:39:37 +0000
I already found the paper!
Thanks Dean and Marc.

The paper is in:
PROGRESS IN PROTOZOOLOGY
Proceedings of VI International Congress of Protozoology Special Congress Volume of ACT A PROTOZOOLOGICA
part I, pp. 31-44, 1982

Kaz

From: Ciliate Molecular Biology <[log in to unmask]> on behalf of Kazufumi MOCHIZUKI <[log in to unmask]>
Date: Friday 7 June 2024 at 16:11
To: "[log in to unmask]" <[log in to unmask]>
Subject: Looking for a classical paper by Peter Bruns

[More ...]
Antibody to Tetrahymena PDD1
Eric S Cole
Fri, 31 May 2024 05:18:59 -0500
Reply
Is there a commercial source for antibody to PDD1?
Barring that, does anyone have a smidgen they could share?

best wishes

Eric
Reply
No Replies
Tetrahymena genome annotation
Shan Gao
Mon, 27 May 2024 12:01:10 +0800
Reply
Dear all, I am writing to share some progress from our recent project. After completing the T2T genome assembly of Tetrahymena thermophila in 2020 (PMID: 32297047), we have started to re-annotate the genome with both transcriptomic and epigenomic data. This intensive re-annotation includes updates on genes, alternative splicing, natural antisense transcripts, and non-coding RNA. Besides bioinformatic analysis, the updated gene models have undergone three rounds of manual curation. We plan to publish the data on the Tetrahymena Genome Database (TGD) in the near future, with the collaboration of Dr. Naomi Stover at Bradley University. For the time being, the data 

[More ...]
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A Call For Abstracts!
Megan Valentine
Wed, 15 May 2024 14:00:52 -0400
Reply
Please begin preparing and sending your poster abstracts for this summers virtual ciliate research conference! The conference will be held July 23rd - 25th at 9 am to 12 pm EST. This virtual conference will have a selection of invited speakers who will be contacted directly and posters for anyone who would like to present! Poster presenters will be able to submit their abstracts in the following research focus areas: Motility (cilia, basal bodies, and flagella); Cell biology; Evolutionary biology; Gene expression; Developmental genomic rearrangement; and Developing undergraduate research. Prizes will be awarded to the best undergraduate and graduate student 

[More ...]
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No Replies
Tetrahymena strains without group I intron?
Connor Alec Horton
Tue, 14 May 2024 11:30:36 -0700
Reply
Hi ciliate community!

I'm a graduate student in Kathy Collins' lab at Berkeley, and we are
looking for strains of T. thermophila lacking the group I intron. Does
anyone have strains of this sort they'd be willing to share?

Thanks for your help!

Best,
Connor
Reply
No Replies
CLIP
Ronald E Pearlman
Wed, 17 Apr 2024 23:47:31 +0000
Reply
I think that I may have asked this previously but it was some time ago so it is time to check again. Has anyone done CLIP or PAR CLIP in Tetrahymena and if so, would you be willing to share your protocol?

Thank you.

Ron

Dr. Ronald E. Pearlman [log in to unmask]<mailto:[log in to unmask]>

Past President and Advisor

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axenic Paramecium tetraurelia
Marcella Cervantes
Mon, 1 Apr 2024 14:59:59 -0400
Reply
Hello Everyone,
I am designing a new lab for our introductory cell and molecular biology
lab. Cells with a slower doubling time than T. thermophila are easier to
maintain, so I am going to try *Paramecium*. Does anyone have an axenic
culture of *P. tetraurelia *to share?
Thank you,
Marcella
Reply
No Replies
Email address for Liz Blackburn
Glenn Herrick
Tue, 26 Mar 2024 19:56:59 +0000
Reply
I need to communicate with her, but have lost contact with her.

From my iPhone X, WhatsApp
Italy 39-349-056-0727
USA 01-801-448-5822
Skype name=gherrick
Call *free* from UT to 801-938-4583
Please only reply to [log in to unmask] or [log in to unmask]
Reply
No Replies
Phagocytosi-deficient lines of Tetrahymena?
Katie Deets
Wed, 13 Mar 2024 21:26:20 +0000
Reply
Hi Ciliate Community,

I am currently on the hunt for various phagocytosis-deficient lines of Tetrahymena (T. thermophila or other). I have the heat-sensitive NP-1 line originally made by Eduardo Orias and have ordered the TtVPS13A knockout line (Haresha S. Samaranayake et al.) from the Tetrahymena Stock Center. But I was wondering if anyone else has any similar lines they would be willing to share.

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1 week left to register for the 2024 Midwest Protozoology Society meeting
Mark Slabodnick
Mon, 11 Mar 2024 13:22:33 -0500
Reply
Hi Everyone,

There is just 1 week left to register for the 2024 Midwest Protozoology
Society meeting being held April 6th at Knox College in Galesburg,
Illinois! If you are in the area, it is a great meeting to bring trainees
and learn about some of the cool work being done in the region or present
their own. Whether you are from the region, or from outside the region but
planning a trip to the zone of totality for the 2024 eclipse, we would love
to see you and hear about your work!

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Feature Papers in Microbial Biology
JUAN CARLOS GUTIERREZ FERNANDEZ
Mon, 26 Feb 2024 11:33:18 +0100
Reply
Dear Colleagues,

As one of the collection co-editors "*Feature Papers in Microbial Biology*",
I would like to announce the possibility of collaborating in this
collection with an article if you consider it appropriate. As you can see
in the attached PDF it is open to manuscripts concerning any kind of
microorganisms (including protists) and on a wide variety of potential
topics (genetics, ecology, physiology, structure, molecular biology,
biotechnology, omics, etc.).
Anyone interested in contributing a manuscript, please let me know.
Thanks in advance.

[More ...]
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No Replies
TurboID
Aaron Turkewitz
Mon, 5 Feb 2024 19:42:17 +0000
Reply
Has anyone experimented with TurboID for proximity labeling in Tetrahymena?

Thanks very much
Aaron

Aaron Turkewitz
Molecular Genetics and Cell Biology
The University of Chicago
Reply
No Replies
2024 Midwest Protozoology Society Meeting
Mark Slabodnick
Mon, 5 Feb 2024 07:00:00 -0600
Reply
Dear colleagues,

I would like to announce that the 2024 Midwest Protozoology Society meeting
will be held on *Saturday April 6th at Knox College in Galesburg, Illinois.*

For those in the region, this is a great opportunity for undergraduate
students to present their work in a poster or talk, meet with colleagues,
and also hear about great research on ciliates and other protists.

[More ...]
Reply
No Replies
Visiting Assistant Professor of Biology (Cell and Molecular)
Wei-Jen Chang
Mon, 29 Jan 2024 22:50:26 -0500
Reply
https://apply.interfolio.com/140105

The Biology Department at Hamilton College invites applications for a
one-year visiting position at the rank of Assistant Professor, beginning
July 1, 2024. We seek candidates with expertise in cellular and molecular
biology to contribute to our core offerings in this area, in consultation
with the department. The courses may also contribute to interdisciplinary
programs in Neuroscience, or Biochemistry and Molecular Biology (BMB). We
are seeking candidates who can demonstrate their experience in teaching or
working with broadly diverse student populations. Your cover letter should
address the ways in which you would further the College's goals of
enhancing the

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Virtual Meeting
Marcella Cervantes
Thu, 25 Jan 2024 16:31:50 -0500
Reply
Hello Everyone,
We will be having a short virtual Ciliate Molecular Biology meeting this
summer highlighting the research of junior scientists, including late
graduate students, postdocs, researchers new to ciliates and non-tenured
faculty. Please post, or forward the announcement below to all who may
be interested. A call for speakers and posters will be sent out in the
coming months.

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No Replies
Tetrahymena cannibalism instead of Mating
Dr. Luisa F. Jimenez-Soto
Thu, 21 Dec 2023 16:56:35 +0100
Reply
Dear All,

Josef did say your community is super friendly... but I had no idea how
much it is. You have been already so welcoming! Thanks!

Here is the story:

I decided to grow T. thermophila in a vegetarian media (NEFF with
Peptone from Soy) (After all, Tetra has to arrive to the 21st century
;-) ). After a couple of generations, when we prepare them for mating in
Starvation media some of them explode and the others will gather around
and cannibalize the dead cells. If this happens, we know automatically
that mating will not occur at all. I

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Reply
Show Replies 1 Reply
Re: Tetrahymena cannibalism instead of Mating
Luisa F. Jimenez-Soto
Fri, 22 Dec 2023 02:48:03 -0500
Dear Eric, Rachel, Giulio, Douglas and Yifan,
Thanks for all the ideas and questions!
Here is the summary from all what you asked and wrote. If I am doing something wrong by posting in here, please let me know. I rather place all the info for other that might have seen something similar or will see something similar in the future. The more brains, the better :-)

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Community participation
Dr. Luisa F. Jimenez-Soto
Thu, 21 Dec 2023 07:10:27 +0100
Reply
Dear Tetrahymena community,

Prof. Josef Loidl gave me this address. He told me that you might be
able to help me with a weird behavior we are observing with our
Tetrahymena thermophila strains.

Since it is my first tome using a e-mail address as a forum, I will
greatly appreciate if someone can explain how this works ;-)

[More ...]
Reply
Show Replies 2 Replies
Re: Community participation
Thomas Doak
Thu, 21 Dec 2023 09:38:11 -0500
Post away! We will see what we can do for you!

Cheers,
Tom

> On Dec 21, 2023, at 1:10 AM, Dr. Luisa F. Jimenez-Soto <[log in to unmask]> wrote:
>
> Dear Tetrahymena community,
>
> Prof. Josef Loidl gave me this address. He told me that you might be able to help me with a weird behavior we are observing with our Tetrahymena thermophila strains.
>
> Since it is my first tome using a e-mail address as a forum, I will greatly appreciate if someone can explain how this works ;-)
>
> I wish you all a great start in

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Re: Community participation
Thomas Doak
Thu, 28 Dec 2023 10:39:12 -0500
One comment I forgot to make: at least for larger ciliates (Oxytricha, Stylonychia) having some calcium in the mating media is quite important. It took me an embarrassingly long time to realize this, having inherited a protocol that used a buffer free of calcium. Pringshams salts have worked well for me. Someone else can comment on the application of this thought to Tetrahymena. 

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PhD studentship in Tetrahymena hormone metabolism
Jan Janouskovec
Wed, 20 Dec 2023 18:31:26 -0500
Reply
Dear ciliate researchers,

We are offering a PhD studentship in hormonal biosynthesis and evolution in Tetrahymena. The application deadline is on January 2, 2024.

https://www.alga.cz/UserFiles/mstefanova/files/2023_PhD_molecular_biology_v1_EH(1).pdf

Please forward to whoever may be interested in this.

Thank you,

Jan

=====
Jan Janouskovec
Group leader.
Institute of Microbiology, Trebon, Czech Republic
Email: [log in to unmask]
Website: https://www.alga.cz/
Reply
No Replies
iCLIP
Ronald E Pearlman
Tue, 19 Dec 2023 00:43:41 +0000
Reply
Has anyone done iCLIP in Tetrahymena and has a protocol that works that they are willing to share? I may have missed something but I have not found anything published for Tetrahymena.

Thank you.

Ron

Dr. Ronald E. Pearlman [log in to unmask]<mailto:[log in to unmask]>

Past President and Advisor

Royal Canadian Institute for Science (RCIS)

University Professor Emeritus and Senior Scholar

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gold particles for Tetrahymena biolistics?
Suzanne Lee
Sat, 9 Dec 2023 01:52:49 +0000
Reply
Hi ciliate colleagues,

I am looking to replenish my supply of gold particles, and had ordered the ones available through Seashell Technologies back in 2015, but it looks like this company is no longer running.

Would anyone mind sharing with me where and under what catalog number they get their gold particles for making Tetrahymena transformants via the gene gun/biolistics?

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Show Replies 1 Reply
Re: gold particles for Tetrahymena biolistics?
Santosh Kumar
Sun, 10 Dec 2023 10:41:24 +0530
Hi Suzanne,
Critter Technology is now selling the same biolistic delivery particles. If
you would like more information or would like to purchase biolistic
delivery particles (e.g. s550d) please e-mail [log in to unmask] or
call 619-993-4755.

Best,

Santosh
NCCS, Pune ( India)

On Sat, Dec 9, 2023 at 8:07 AM Suzanne Lee <[log in to unmask]> wrote:

> Hi ciliate colleagues,
>
> I am looking to replenish my supply of gold particles, and had ordered the
> ones available through Seashell Technologies back in 2015, but it looks
> like this company is no longer running.
>
> Would anyone mind sharing with me where

[More ...]
ASCB Ciliate Lunch
Brian Bayless
Fri, 17 Nov 2023 12:35:16 -0500
Reply
Hi ASCB going ciliate community,

I hope everyone is doing well this year. The ASCB meeting is in Boston and I would like to organize a gathering for all of our community that are attending. I am aiming for Monday the 4th around 12:30. If you, or any of your students/postdocs, would like to attend please fill out the following Google form or email me at [log in to unmask] no later than Monday the 27th of November so I can secure a lunch spot.

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Open call for new group leaders at the I2BC (France)
Mireille BETERMIER
Thu, 9 Nov 2023 14:13:31 +0100
Reply
Dear colleagues of the ciliate community,

The Institute for Integrative Biology of the Cell (located in
Gif-sur-Yvette, France) is currently seeking new group leaders for its
Department of Genome Biology. The call is open to all relevant topics,
with a particular emphasis on RNA biology, chromatin organization,
genome stability, and gene expression. The application deadline is
January 15th, 2024.

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Special issue "Diversity, Ecology and Genetics of Ciliates"
Alexey Potekhin
Tue, 10 Oct 2023 17:45:05 +0200
Reply
Dear ciliate biologists,

We would like to invite you to contribute your manuscripts to the Special
Issue *“**Diversity, Ecology and Genetics of Ciliates*” announced by*
'Diversity' (MDPI)*. We are the guest editors of this special issue (
https://www.mdpi.com/journal/diversity/special_issues/03465W3H94).
Diversity (Impact
Factor 2.4, ranking Q2 in JCR) is a fully open access journal of MDPI. The
review process is quick and professional, and the publication time, if the
article is accepted, aims to be within 1-3 months after submission.

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Frontiers in Epigenetics and Epigenomics: would you like to contribute to "Current Insights in Epigenetics and Epigenomics"?
Simran Bhullar
Fri, 15 Sep 2023 17:49:02 +0000
Reply
Dear Ciliate Community Members,

I received an email from a recently launched journal from the Frontiers series, this one is called, "Frontiers in Epigenetics and Epigenomics."

They are putting up a collection, Current Insights in Epigenetics and Epigenomics. Please find the information in the email below.

I know we have had special issues, as in the Journal Microorganisms, edited by Cristina Miceli, Adriana Vallesi and Ron Pearlman in 2022, then we had another special issue in Journal Genes in 2019. This is not specific to ciliate biology, so I feel there should be a representation of the ciliate community.

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TetraMania
Ronald E Pearlman
Fri, 14 Jul 2023 19:21:13 +0000
Reply
Colleagues: Many of you are aware that we have been working on a www based application to identify and analyze protein/protein interactions, common domains in proteins, and other things. The application is called TetraMania and it was developed in collaboration with colleagues at University of Toronto who developed GeneMania for similar analysis with yeast and now supporting a number of other organisms. TetraMania is free standing and not part of the GeneMania application. The most recent update of GeneMania, a 2018 publication is attached fyi. 

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Cell Cycle RNAseq data
Marcella Cervantes
Mon, 10 Jul 2023 20:08:33 -0400
Reply
Hello Ciliate Community,
Seeing many of you in France after such a long time was terrific. This
email is a follow-up to some questions. If you have no interest in the *T.
thermophila* RNAseq cell cycle data, you can stop reading now. No offense
taken.
A few of you had questions about where to find the cell cycle paper and
data. The paper can be found at
https://www.molbiolcell.org/doi/10.1091/mbc.E22-08-0326. There is an
incorrect accession number for the raw data which we are tending to. The
correct NCBI GEO accession number for the raw data is GSE209619, or you can
search for

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Hans Joachim Lipps
Postberg, Jan
Fri, 30 Jun 2023 20:22:30 +0000
Reply
Dear Colleagues,
I regret to inform you that our valued former colleague Hans Lipps has passed away. I traveled to Tübingen today to visit him and explore the Paleolithic caves together, as we had planned last fall. However, I received the sad news that Hans has been in the hospital for several days. Despite his condition, I had hoped to visit him if possible. Unfortunately, I just received the sad news that Hans Lipps passed away yesterday.
Hans Lipps was a highly respected member of our scientific community and a mentor to many. His unexpected death leaves a huge void

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Ciliate Molecular Biology Meeting Poll and Call for Ciliate Advisory Board Members
Pearson, Chad
Wed, 7 Jun 2023 21:09:42 +0000
Reply
Dear Ciliate Molecular Biology Community,

We are looking forward to seeing you all either in person or online in France at the end of June.

In preparation for the upcoming and future Ciliate Molecular Biology meetings, the Ciliate Advisory Board is asking for your feedback:

We have three questions/sections to determine your:
1) interest in future meeting attendance based on geographic location
2) interest in hosting/organizing the 2025 Ciliate Molecular Biology meeting
3) nominations (self and others) for Ciliate Advisory Board membership

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Post-doc and PhD student positions in Warsaw, Poland
Jacek Nowak
Mon, 15 May 2023 13:32:47 +0200
Reply
Dear colleagues,

My laboratory at the Institute of Biochemistry and Biophysics of the Polish
Academy of Sciences in Warsaw is looking for a candidate for a 3-year
postdoctoral fellowship (can be started immediately or by arrangement) and
a candidate for a 4-year doctoral program (starting in October 2023) to
study non-coding transcription in Paramecium tetraurelia.

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Klobutcher lab materials
Klobutcher,Lawrence A.
Wed, 10 May 2023 16:29:16 +0000
Reply
Dear Colleagues:
As many of you know, I retired three years ago, but have maintained a small lab since that time. The lab has allowed me to maintain live cultures of Euplotes crassus, frozen stocks of Tetrahymena thermophila, and reagents and materials generated during my past research.

I have decided to stop my laboratory operation effective September 1 of this year. If anyone is interested in ciliate strains, materials, or items related to culturing ciliates, please contact me. I will do my best to accommodate any requests.

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urgent - help needed to identify/characterize a ciliate affecting new coral recruits of at risk Florida Keys corals spawned in captivity
Yanong, Roy P.E.
Fri, 21 Apr 2023 15:00:10 +0000
Reply
Hello,

I've been added to this listserve thanks to the kindness of Dr. Jacek Gaertig, and hope that one or more of you can help me and my colleagues out with a potentially problematic ciliate affecting Florida Keys coral recruits from broodstock spawned in captivity.

I am an aquatic veterinarian/ faculty at the University of Florida, based in the School of Forest, Fisheries, and Geomatics Sciences, working with the Coral Rescue project, an Association of Zoos and Aquariums (AZA) project under the Florida Fish and Wildlife Conservation Commission's purview. Stony Coral Tissue Loss Disease (see: https://www.coris.noaa.gov/activities/stony_coral_tissue_loss_disease/) has caused significant mortalities

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Re: urgent - help needed to identify/characterize a ciliate affecting new coral recruits of at risk Florida Keys corals spawned in captivity
Yanong, Roy P.E.
Fri, 21 Apr 2023 15:29:29 +0000
As a follow up to my first email -see below - we do have some videos and photomicrographs (not the best quality, however) but based on our "first impression" the ciliate looks very much like the ciliate in Figure 1C of Randall et al 2015. Ciliates associated with signs of disease on two Caribbean corals. Coral Reefs 34:243-247. I wasn't sure if I had permission to attach a copy, so I didn't. 

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CMB2023 abstract deadline is extended to April 25
Kazufumi MOCHIZUKI
Thu, 13 Apr 2023 15:30:56 +0000
Reply
Dear Colleagues,

For those of you who wish to present your work in the Ciliate Molecular Biology 2023 Meeting but are too busy to prepare an abstract in a few days, we have decided to extend the deadlines for registration and abstract submission.

Now the new deadline for early bird registration and abstract submission is April 25th, 2023

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CMB2023 discounted registration until April 15
Kazufumi MOCHIZUKI
Tue, 4 Apr 2023 12:21:09 +0000
Reply
Dear Ciliate Folks,

This is just a reminder that the early-bird registration dead line for the Ciliate Molecular Biology 2023 meeting is in two weeks (April 15). We will have an exciting program in a lovely city! In case you haven’t checked out the meeting website yet, please visit https://www.ciliate-meeting-2023.com/en/ and consider joining the meeting.

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Lab Coordinator Position @ Hamilton College
Wei-Jen Chang
Tue, 14 Mar 2023 22:28:09 -0400
Reply
Dear colleagues,

The full job description can be found here:
https://apply.interfolio.com/122568

The Biology Department at Hamilton College invites applications for a
Laboratory Coordinator position, beginning June 1st 2023. We seek
candidates to teach two biology laboratory sections per year, order
supplies and track the departmental budget, help to maintain general
departmental facilities and equipment, serve as the departmental chemical
hygiene officer, and assist with other departmental functions. Your cover
letter should address your teaching experience and area(s) of expertise in
Biology, record-keeping experience, attention to detail, and a demonstrated
ability and willingness to be proactive and learn new skills. Equivalent


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Registration site for Ciliate Molecular Biology 2023 Meeting
Kazufumi MOCHIZUKI
Tue, 7 Mar 2023 15:41:41 +0000
Reply
Dear colleagues,

We are pleased to inform you that the registration website for the Ciliate Molecular Biology 2023 Meeting is now open (https://www.ciliate-meeting-2023.com/en/). Please spread the information. The early registration and abstract submission deadline is April 15 (very soon!). So, please start your registration process and abstract preparation as soon as possible. Also, please start arranging your travels and hotels at your earliest convenience (to save your travel cost). Please note that travels and accommodations are NOT included in the registration.

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change of Email address / Wilfried Pauli / [log in to unmask]
Wilfred Pauli
Fri, 24 Feb 2023 14:00:32 +0100
Reply
Dear Sir or Madame,

My Email address has changed.

Currently I am on your email distribution list with the Email-address:
[log in to unmask]

I would like to stay on the mailing list and would appreciate it if you
would change the address and contact me in the future under the

new address: [log in to unmask]

Many thanks in advance and best regards

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2023 Midwest Protozoology Society Meeting
Mark Slabodnick
Tue, 21 Feb 2023 21:51:31 -0600
Reply
Dear colleagues,

I would like to announce that the Midwest Protozoology Society will return
to having annual meetings! Naomi has (at least temporarily) passed me the
hosting torch and this year, in person, the meeting will be held on *Saturday
April 22nd at Knox College in Galesburg, Illinois.*

For those in the region, this is a great opportunity for undergraduate
students to present their work in a poster or talk, meet with colleagues,
and also hear about great research on ciliates and other protists.

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Re: 2023 Midwest Protozoology Society Meeting
Mark Slabodnick
Mon, 10 Apr 2023 07:21:59 -0500
Dear colleagues,

I am *extending the deadline for registration until Wednesday April 12th*!
If you are interested in attending the Midwest meeting on Saturday April
22nd held at Knox College, or know someone that might be, there is still
time to sign up!

*Registration Link:* https://forms.gle/EBiTBRvR9YZLPTi6A

*Registration will cost $10 for students and $30 for postdocs/faculty* to
cover the cost of lunch, snacks, and coffee that will be provided. *You
will receive a separate email from me after you register with payment
details*.

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Visiting Assistant Professor of Biology (Ecology)
Wei-Jen Chang
Sat, 11 Feb 2023 09:15:10 -0500
Reply
https://apply.interfolio.com/121085

Description

The Biology Department at Hamilton College invites applications for a
visiting position at the rank of Assistant Professor, beginning July 1,
2023. This is a one-year position with the potential for a one-year
renewal. We seek candidates to teach Ecology with an associated lab,
Introductory Biology, and related courses. We are seeking candidates who
can demonstrate their experience in teaching or working with diverse
student populations. Your cover letter should address the ways in which you
would further the College's goal of building a diverse and inclusive
educational environment.

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Ciliate Molecular Biology 2023 Meeting, June 26-29, 2023, Montpellier, France
Kazufumi MOCHIZUKI
Thu, 26 Jan 2023 10:39:18 +0000
Reply
Dear colleagues,

On behalf of the organizers, we are sending you the first announcement of the Ciliate Molecular Biology 2023 Meeting which will be held from June 26 to 29, 2023 in Montpellier, France. This will be a long-awaited opportunity to get together in person and we hope that many of you will attend the meeting on site. It will be also possible to join the meeting remotely, as we will organize the meeting with a hybrid format. You can find the list of confirmed invited speakers, the latest draft program, and other information in the attached pdf. Please spread

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Re: Ciliate Molecular Biology 2023 Meeting, June 26-29, 2023, Montpellier, France
Asai, David J
Thu, 26 Jan 2023 14:27:23 +0000
I’m following Kazu’s happy message with a much sadder one.

I’m sure many of you already saw the news, but I wanted to reach out the entire community. Dave Allis passed away January 8. You can read many tributes to Dave on the internet. I communicated with Jim Forney last night, and Jim gave my feeling words: “Dave did great work and was always helpful to anyone using ciliates experimentally.”

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Re: Ciliate Molecular Biology 2023 Meeting, June 26-29, 2023, Montpellier, France
Kazufumi MOCHIZUKI
Thu, 26 Jan 2023 15:15:04 +0000
Dear David, Dear all,

It is very sad news indeed. We plan to have the memorial session in Day 4 (June 29), which will be dedicated to Dave Allis and Martin Gorovsky.

Kaz

From: Asai, David J <[log in to unmask]>
Date: Thursday, 26 January 2023 at 15:27
To: Kazufumi MOCHIZUKI <[log in to unmask]>, [log in to unmask] <[log in to unmask]>
Subject: Re: Ciliate Molecular Biology 2023 Meeting, June 26-29, 2023, Montpellier, France
I’m following Kazu’s happy message with a much sadder one.

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Re: Ciliate Molecular Biology 2023 Meeting, June 26-29, 2023, Montpellier, France
Kazufumi MOCHIZUKI
Thu, 26 Jan 2023 15:38:39 +0000
It will happen on Day 3 (June 28). Sorry for my mistake.
Kaz

From: Ciliate Molecular Biology <[log in to unmask]> on behalf of Kazufumi MOCHIZUKI <[log in to unmask]>
Date: Thursday, 26 January 2023 at 16:15
To: [log in to unmask] <[log in to unmask]>
Subject: Re: Ciliate Molecular Biology 2023 Meeting, June 26-29, 2023, Montpellier, France
Dear David, Dear all,

It is very sad news indeed. We plan to have the memorial session in Day 4 (June 29), which will be dedicated to Dave Allis and Martin Gorovsky.

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change of email
Muhittin ARSLANYOLU
Mon, 2 Jan 2023 10:10:23 +0000
Reply
Dear all,

Would you help me to change my email address as [log in to unmask] ?

Best regards

Prof.Dr.Muhittin Arslanyolu
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Tenure Track Position at Saginaw Valley State University
Brian Bayless
Tue, 13 Dec 2022 12:10:33 -0500
Reply
Hi Everyone,

Sylvia Fromherz, from Saginaw Valley State University asked me to let you all know that they are hiring a Molecular Biologist/Geneticist for a tenure-track faculty position at the assistant professor level. They would welcome a ciliate biologist to their department as Sylvia has recently begun some ciliate related research in her own lab! The job posting is here:

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Research MS opportunities for undergrads/postbaccs
Suzanne Lee
Tue, 6 Dec 2022 19:07:14 +0000
Reply
Dear colleagues,

My Department at Western Washington University offers a research Masters of Science degree in Biology and research labs that are accepting students are listed in the attachment (this includes my and Nick Galati’s labs, where Tetrahymena is being used as a model system!). Please share with any potentially interested students you know or are in your networks.

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Tetrahymena transformation by electroporation
Kazufumi MOCHIZUKI
Mon, 21 Nov 2022 09:17:32 +0000
Reply
Dear all,

I am writing to ask you to share your experiences on transformation of Tetrahymena cells by electroporation.

I was frequently asked by non-Tetrahymena researchers that why we use biolistic transformation instead of electroporation, and I always told them that we can use electroporation only for some limited types of genetic manipulation because electro-transformation somehow only works with cells at late conjugation stages (= the new macronucleus) in Tetrahymena.

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Re: Tetrahymena transformation by electroporation
Kazufumi MOCHIZUKI
Fri, 9 Dec 2022 17:27:39 +0000
Dear all,

This is just a follow up of my previous post about electroporation.

Yasuhiro Fukuda at Tohoku University, Japan kindly replied me that he is constantly using electroporation for introducing transgenes into the macronucleus using starved Tetrahymena cells. He said he basically uses the protocol described in Iwamoto et al. (2009) Curr. Biol.19, 843–847 and Nakagawa, et al. (2008) Cell Struct. Funct. 33, 151–62 with some minor modifications. Transformation worked in ~90% of cases in his hands and in most of the failed cases, he succeeded to get transformants in his second trials with the same condition and constructs.

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Re: Tetrahymena transformation by electroporation
Ronald E Pearlman
Fri, 9 Dec 2022 19:24:15 +0000
Dear all: Thank you to Yasuhiro and to Kaz for this. The protocols or contacting Yasuhiro as per Kaz's e-mail should be the information of interest.

For anyone interested, the paper mentioned in the e-mail is now published in the special ciliate issue of Microorganisms and it contains the protocol and some data from electroporation transformation of starved cells. The paper can be accessed on the Website: at https://www.mdpi.com/2076-2607/10/12/2426 and a PDF
Version is available at: https://www.mdpi.com/2076-2607/10/12/2426/pdf. Good luck.

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ASCB Ciliate Lunch
Brian Bayless
Mon, 7 Nov 2022 11:19:57 -0500
Reply
Hi ASCB going ciliate community,

I hope everyone is doing well and the transition to a world living with COVID is as good as it can be. The ASCB meeting is back in person this year and I would like to organize a gathering for all of our community that are attending. If you, or any of your students/postdocs, would like to attend please fill out the following Google form or email me at [log in to unmask] no later than Monday the 14th of November so I can secure a lunch spot.

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Leptomycin B/Nuclear export inhibitor?
Suzanne Lee
Thu, 27 Oct 2022 17:25:33 +0000
Reply
Hi colleagues,

I’m curious if anyone has experience (either positive or negative) in using leptomycin B as a potential nuclear export inhibitor in Tetrahymena? Any leads would be welcome!

with thanks in advance,
Suzanne

----
Suzanne R Lee, PhD
Associate Professor
Biology Department
Western Washington University
Website: biology.wwu.edu/people/lees65<http://biology.wwu.edu/people/lees65>
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Special Ciliate issue-Microorganisms-UPDATE
Ronald E Pearlman
Tue, 27 Sep 2022 18:41:57 +0000
Reply
Dear all: The deadline for submission of manuscripts to the special ciliate issue of Microorganisms was September 30, 2022. A number of colleagues have informed us that they have manuscripts in preparation but are unable to complete and submit them by September 30.

We have been able to get agreement from the publisher to extend the deadline for submissions to November 30, 2022. We hope that some of you will be able to meet this deadline and submit a manuscript by November 30. There is no limit to the number of manuscripts that we can accept. Criteria for acceptance are

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International Symposium on Ciliate Biology 2022-Invitation
renu gupta
Sun, 25 Sep 2022 10:01:33 +0000
Reply
Greetings from India!!

In commemoration of Centenary Celebrations of University of Delhi, Delhi, India, Acharya Narendra Dev College and Maitreyi College, in collaboration with INSCR (Indian Network for Soil Contamination Research) are organizing an online 3rd “International Symposium on Ciliate Biology 2022 (ISCB 2022)” on November 08, 2022.

The symposium will focus on several aspects of ciliates such as their diversity, ecology, evolution, systematics, genomics, epigenetics, barcoding, proteomics, ciliates and industry, ciliates as model organisms in UG teaching and in research.

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Postdoctoral position available...
Mark Winey
Mon, 19 Sep 2022 04:13:31 +0000
Reply
Hello everyone,

I am forwarding this on behalf of James Letts here at UC Davis. Hopefully you saw James talk about this work at last summer's on-line ciliate meeting

Best,

Mark
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Ciliate Advisory Board election results
Sean Taverna
Fri, 19 Aug 2022 14:12:21 -0400
Reply
Dear ciliate community,
This year's two new Ciliate Advisory Board members are *Laura Landweber *and
*Shan Gao*. Their 3-year terms begin next month, and we will reach out to
them to schedule a CAB zoom meeting soon. We extend a big thank you to each
of our five outstanding candidates for volunteering, and to everyone for
participating in the elections - every vote mattered!

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Another tenure-track faculty search open!
Suzanne Lee
Wed, 17 Aug 2022 22:56:08 +0000
Reply
Dear Colleagues,

A second open search in my Dept may be of interest to you or those you know:

The Biology Department in the College of Science and Engineering has approximately 30 faculty whose expertise spans a broad range of biological disciplines. The department, college, and university are devoted to creating an equitable environment for students and staff and are engaged in the process of addressing institutional racism and other oppressive biases. We especially encourage applications from women, people of color, people with disabilities, veterans, and other candidates from historically excluded backgrounds and underrepresented experiences.

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2022 Ciliate Advisory Board Elections - PLEASE VOTE TODAY!
Pearson, Chad
Mon, 15 Aug 2022 16:51:36 +0000
Reply
Hello all

We have five candidates for two slots on the Ciliate Advisory Board. Thank you to all candidates for their dedication to our community!

Please click on the below link to place your vote.
https://forms.gle/4DxnU4Qu7CVfVeq97

A few notes:
-Please vote only once. Emails are registered to limit replicate voting. When tallying votes, we will not associate your email with your votes.
-Please vote for only two candidates.
-Voting will close at midnight (eastern) on Wednesday, August 17, 2022. Please vote now.

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Ciliate Advisory Board nominations/election timeline
Sean Taverna
Wed, 3 Aug 2022 17:02:59 -0400
Reply
Dear Ciliate community,

First of all, thank you to everyone who was able to attend our virtual
meeting last week on 7/27. It was exciting to interact with all of you and
hear about your new research during the talks, posters, and breakout room
chats. There was also a lot of excitement about our upcoming in-person
meeting next year in France, scheduled for June 25th-29th in 2023
(organized by Kaz Mochizuki, Brian Bayless, Shan Gao, and Lydia Bright).
Save the date!

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Biochemistry Tenure-track Faculty Search at Western Washington University
Suzanne Lee
Wed, 27 Jul 2022 23:45:42 +0000
Reply
Dear colleagues,

So great to see many of you today at the Ciliate Molecular Biology meeting, and looking forward to future meetings with this terrific community.

In case there are job seekers out there who are looking for a tenure track faculty position at a primarily undergraduate institution (with some Masters students) in the US, please see and/or share this: https://hr.wwu.edu/careers-faculty?job=499995&fbclid=IwAR14qI3r-lyrvI0kDfa764RlmqymzErsKAu-l6r-e31njnPiACRYQGh4Wv0.

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Microorganisms-Special Issue-please consider publication and support
Ronald E Pearlman
Wed, 6 Jul 2022 13:44:01 +0000
Reply
Dear Colleagues: This is an update to the Special Issue on Ciliates for the Journal Microorganisms that Cristina Miceli, Adriana Vallesi, and I are co-editing. We have a good number of manuscripts already received and published or in process for this issue. The original date for submission was December 31, 2021. We have however recently worked with the publishers and have arranged for the submission deadline to be moved to September 30, 2022. We have received a number of commitments for submissions by this date. This note is to provide updated information and to encourage colleagues to submit strong research 

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Postdoctoral positions in my lab
Laura Katz
Mon, 27 Jun 2022 09:19:07 -0400
Reply
Greetings all, i'd be grateful if you could share the message below as I am
looking to hire two postdocs in the coming months.
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Temporary CURE Instructor Job Opportunity in Boulder
Kristin Ann Moore
Wed, 8 Jun 2022 15:56:09 +0000
Reply
Hi Ciliate Community,

Could you please share the following job ad link and summary to anyone you think may be interested in teaching a CURE course (5-6 sections) for Fall 2022 at University of Colorado Boulder? This is a temporary, 1 semester full-time position. The job requirement requests a PhD, but we can be flexible if someone has teaching experience but not a PhD.

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2022 Virtual Ciliate Molecular Biology Meeting - July 27 9-2p EST
Pearson, Chad
Fri, 3 Jun 2022 14:57:45 +0000
Reply
Dear Ciliate Community

We are pleased to announce a (mostly solidified!) schedule for the 2022 Virtual Ciliate Molecular Biology Meeting that will occur on Wednesday, July 27, 2022 from 9:00a-2:00p (EASTERN TIME).

REGISTRATION

Why not, register right now! Please do not wait.

REGISTER HERE<https://forms.gle/YbFTkLdpQaQY5Xp5A> (or copy URL into Browser: https://forms.gle/YbFTkLdpQaQY5Xp5A)

Registration by participants allows us to report our attendance for future meeting funding.

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Re: 2022 Virtual Ciliate Molecular Biology Meeting - July 27 9-2p EST
Pearson, Chad
Tue, 5 Jul 2022 20:55:03 +0000
Hello all

We are sending along one last email asking you to please register for the upcoming 2022 Virtual Ciliate Molecular Biology Meeting. We have a great lineup of speakers (please see below) and poster presentations, and nearly 100 registered participants. Please join us.

The deadline for registration is July 19, 2022.

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new deadline for special issue on Paramecium
Judith Van Houten
Wed, 1 Jun 2022 14:02:08 -0400
Reply
Dear All,

Good news:  The deadline for this issue has been moved back to November 30!

I am writing to invite you to contribute an article to a special issue
of Microorganisms (ISSN 2076-2607, IF 4.152) on /Paramecium/ as a modern
model system. My purpose here is to specifically showcase /Paramecium/
so that new scientists and granting agencies will use and support this
system. I very much hope that you will participate with a short article
or short review, or whatever you would like to contribute to make
visible our /Paramecium/ community.

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"Why Ciliates?" video?
Suzanne Lee
Sun, 29 May 2022 22:24:45 +0000
Reply
Dear Colleagues,

I use the “Why Ciliates?” video that was produced several years ago to engage my students and highlight our model system on my website, but was surprised to find today that the video is no longer accessible on Vimeo, where it was hosted for the longest time. Does anyone have a copy of the video file itself that I might use for my website to replace the broken link?

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Re: "Why Ciliates?" video?
Jacek Nowak
Mon, 30 May 2022 09:56:01 +0200
Dear Suzanne,

Here it is:
https://www.youtube.com/watch?v=ICfd5glXcaY

Best,
Jacek K. Nowak, PhD
VIRTUAL CILIATE MOLECULAR BIOLOGY MEETING - JULY 27, 2022 - PLEASE SAVE THE DATE
Pearson, Chad
Fri, 13 May 2022 19:16:15 +0000
Reply
Hello Ciliate Community,

The Ciliate Advisory Board (Mireille, Marcella, Jacek, Sabrice, Chad, and Sean) would like to invite everyone to attend this summer's 2022 Virtual Ciliate Molecular Biology Meeting - "the last one, right?". This will occur on July 27, 2022. Details are in the works and will be communicated to you all very soon. We intend to have a select number of talks, as many posters as everyone can dream up, and a community meeting. Please mark your calendars.

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cysts
Marcella Cervantes
Wed, 4 May 2022 13:53:15 -0400
Reply
Hello fellow ciliate researchers,
Thanks to nice phylogeny work by Becky Zufall, we can say that we have
isolated what is a newly identified Tetrahymena species! I am looking for a
collaborator who has the technical ability to image ciliate cysts and has
time to complete the imaging in the immediate future. If you know how the
cysts should be prepared, but do not have time right now, any suggestions
or protocols would be greatly appreciated!
Thank you,
Marcella
Reply
Show Replies 1 Reply
Re: cysts
Gregory A Antipa
Wed, 4 May 2022 23:51:58 +0000
Marcella,

For SEM, put your cysts in one drop of whatever media they are in and place the coverslip with the drop hanging over a bottle of 2% osmium oxide for 2 to 5 minutes and rinse them in distilled water and prepare them as you like. Further in the attached article there are details.

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special edition Paramecium
Judith Van Houten
Mon, 4 Apr 2022 16:34:12 -0400
Reply
Dear All,

I am writing to invite you to contribute an article to a special edition
of Microorganisms (ISSN 2076-2607, IF 4.152) on /Paramecium/ as a modern
model system. I very much hope that you will participate with a short
article or short review, or whatever you would like to contribute.  It
is very important to re-create or reinforce our research communities
that have been supportive of our work on /Paramecium/, especially as we
emerge from working separately during the pandemic. I have benefited
personally from colleagues and want to pay back to them and pay forward
to new investigators.

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No Replies
Postdoctoral position in Tetrahymena genetics - Institute of Microbiology, Czech Republic
Jan Janouskovec
Tue, 15 Mar 2022 06:39:00 -0400
Reply
Dear ciliate community,

We are hiring a postdoctoral researcher in Tetrahymena genetics at the Institute of Microbiology, Czech Republic (Centre Algatech in Trebon). Our group is studying the metabolism in mitochondria and chloroplasts in a variety of protistan models: https://www.alga.cz/en/c-874-jan-janouskovec-s-group.html. The postdoctoral researcher will focus on understanding mitochondrial phospholipid synthesis in Tetrahymena thermophila by using subcellular protein localization, genetic knockout, subcellular fractionation, stable isotope labelling, and other methods. We are looking for someone with previous experience in Tetrahymena genetics, an outgoing personality and passion for cell biology.

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No Replies
Tetrahymena Stock Center transition and possible disruption of services
Doug Chalker
Fri, 11 Mar 2022 16:31:39 -0500
Reply
Dear Ciliate Research Community.

The Tetrahymena Stock Center is in the process of transitioning from its original home at Cornell University to its future home at Washington University in St. Louis. During this transition over the next few months, we aim to maintain services, but anticipate possible delays in fulfilling requests. We would like to thank the community for their patience and continuing support during the transition.

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No Replies
Visiting Assistant Professor of Microbiology
Wei-Jen Chang
Sun, 30 Jan 2022 08:57:17 -0500
Reply
https://apply.interfolio.com/102045

Visiting Assistant Professor of Microbiology
Hamilton College: Hamilton College Faculty
Location
Clinton, NY
Open Date

Jan 28, 2022
Description

The Biology Department at Hamilton College invites applications for a
one-year position at the rank of Visiting Assistant Professor, beginning
July 1, 2022. We seek candidates to teach a lecture + lab course in
Microbiology, and three additional lecture or lab teaching credits that
complement the department's offerings. These may include: an introductory
biology lecture course (1 credit), a lecture + lab course (2 credits), a
non-majors lecture course (1 credit), an upper-level seminar (1 credit), or
supervision of senior

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No Replies
Assistant Professor of Instruction in Microscopy
Wei-Jen Chang
Sun, 30 Jan 2022 08:54:38 -0500
Reply
https://dossier.interfolio.com/apply/102044

Assistant Professor of Instruction in Microscopy
Hamilton College: Hamilton College Faculty
Location
Clinton, NY
Open Date

Jan 28, 2022
Description

The Biology Department at Hamilton College invites applications for a
continuing non-tenure-track faculty position in microscopy, at the rank of
Assistant Professor of Instruction, beginning July 1, 2022. The successful
applicant will support the undergraduate research programs of departments
in the Taylor Science Center that use the Microscopy and Imaging Facility
(MIF) and teach the equivalent of 3–4 courses per year that integrate
microscopy into the Biology curriculum (including contributions to Biology
lab courses) and that demonstrate the principles

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Integrated Taxonomy of Protists: Morphology, Genes, and Symbionts: last call for submission
Alexey Potekhin
Mon, 24 Jan 2022 13:19:35 +0300
Reply
Dear Colleagues, I am sending the last call inviting you to contribute to the Special Issue of  Diversity “Integrated Taxonomy of Protists: Morphology, Genes, and Symbionts” ( https://www.mdpi.com/journal/diversity/special_issues/taxonomy_protists ) which we guest edit with Dr. Martina Schrallhammer. The official deadline for submission of the provisional title, list of authors and abstract to the Special Issue is  January 31, 2022 , however, the manuscript submission link will be kept open till March 31, 2022 . This Special Issue aims to highlight the studies that address the diversity of protists, provide integrative characterization of protist species using genetic markers or genomic data, or describe 

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opportunity to promote Paramecium
Judith Van Houten
Mon, 17 Jan 2022 09:34:45 -0500
Reply
Dear All

I am asking all of you who are interested in promoting your work on
/Paramecium/ to consider publication in the special issue of
Microorganisms by MDPI.  Please let me know if you have any questions.
Best wishes and stay well!
Judith Van Houten

--
Judith Van Houten, PhD
University Distinguished Professor Emerita
Reply
No Replies
encystment in protists
Galo Garcia
Fri, 14 Jan 2022 11:43:43 -0800
Reply
Hi all,

I'm starting a project to understand how encystment protects protists from
harsh environments. One of the organisms that interests me is tetrahymena
rostrata. Do you know where I might acquire this species in a reasonable
time frame? ATCC has it, but it would take 3 months to ship. I'm hoping to
get started sooner. Any suggestions would be much appreciated! I'm also
open to suggestions for protists that undergo fast encystment and
excystment ( within a period of 24 hours). Thank you and have a great
weekend!

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Do you have Tetrahymena GFP lines that my class can use?
Lydia Bright
Tue, 11 Jan 2022 16:18:10 +0000
Reply
Hello ciliate colleagues,

I would like to switch my cell bio lab from C. elegans GFP lines to Tetrahymena this year, and I need your help. We usually have each student analyze a C. elegans GFP-tagged line of 'their own', characterizing it through Westerns, fractionation, Real-Time PCR, and confocal microscopy.

Does anyone have any GFP-tagged Tetrahymena cells up and running in their lab that they would be willing to share during this coming semester? We are happy to pay the cost of shipping them to the Hudson Valley.

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funded Masters degree opportunity
Suzanne Lee
Wed, 15 Dec 2021 23:42:44 +0000
Reply
Dear Ciliate Colleagues,

I am actively recruiting for a Masters student in Biology who might be interested in joining my research program. This position would be a good fit for a student who is graduating this year with a bachelors degree, or recently graduated in the past couple years, and seeking research experience within a formal program but not yet ready for a PhD program. Ideal candidates should have some biology or chemistry lab experience involving wet lab experiments or computational experience in sequence analysis or microscopy image analysis, but prior formal research experience is not required.

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rRNA depletion
Kazufumi MOCHIZUKI
Tue, 14 Dec 2021 09:20:13 +0000
Reply
Dear Ciliate Community Members,

If some of you have any experience in removing ribosomal RNAs from Tetrahymena total RNA using Ribo-Zero or its related methods, could you share your experience with me? I want to sequence non-poly(A) RNAs so oligo(dT)-based enrichment does not work for my purpose. Any information from your successful and/or unsuccessful experience would be useful for me.

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Re: rRNA depletion
Estienne Swart
Tue, 14 Dec 2021 11:25:10 +0100
Hi Kaz,

Can't comment on Ribo-Zero or Tetrahymena, but I do recall as a student
in Laura's lab having Oxytricha libraries treated with RiboMinus.
Unfortunately the effect was slightly RiboPlus. Whenever we've looked
into oligos used in commercial kits, they've looked pretty useless due
to ciliate rRNAs being so divergent from the usual suspects they target.
For a different ciliate my technician also tried a custom rRNA
subtraction method with probes we designed to target the most abundant
contaminants from a prior round of subtraction free ribosome profiling.
Unfortunately, no luck trying this either.

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Two tenure-track faculty positions open
Suzanne Lee
Fri, 29 Oct 2021 04:57:56 +0000
Reply
Dear colleagues,

My Department is hiring for two new faculty at the Assistant Professor level this year; see advertisement and links below. In addition to two colleagues with Tetrahymena expertise (myself and Dr. Nick Galati), our faculty also include other individuals who study other ciliates and protists, including Dr. Robin Kodner and Dr. Brady Olson. I would love to see applications from more ciliate folks. Happy to answer any questions about the positions and my institution.

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Ph.D. scientist position at TetraGenetics an AbCellera company
Janna Bednenko
Tue, 19 Oct 2021 19:09:45 +0000
Reply
Dear ciliate molecular biology community,
TetraGenetics an AbCellera company has 1 position open for a Ph.D. level scientist with Tetrahymena experience (scientists with experience in genetics of other ciliates will also be considered). TetraGenetics is located in Boston, MA area, and scientists from other states and countries are encouraged to apply. This is a great opportunity to join a drug discovery company in order to contribute to developing new medicines.
Please find the position description attached. I would appreciate if you could share this information with graduate students and postdocs who may be interested in joining Genetics group at TetraGenetics.

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An invitation to contribute...
Mark Winey
Tue, 19 Oct 2021 18:23:46 +0000
Reply
Dear Colleagues,

I am pleased to tell you that the members of my lab and I have been invited to submit an article tentatively entitled "New Insights from Ciliates" for Volume 38 of the Annual Review of Cell and Developmental Biology. Our manuscript is due in January.

We welcome your input and learning about your recent work. Please send me your thoughts and links to your recent papers or manuscripts at bioRxiv to:

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faculty position at U Houston
Zufall, Rebecca A
Tue, 12 Oct 2021 14:43:33 +0000
Reply
My department is hiring in Evolution and/or Ecology with emphasis on Climate Change. It’s a great department, and I’m happy to talk to anyone about it.

-Becky

Faculty Position in Ecology and Evolution at the University of Houston

The Ecology and Evolution Division of the Department of Biology
and Biochemistry at the University of Houston invites applications
for a tenure-track faculty position at the rank of Assistant
Professor. Applicants with research interests and a record of
accomplishment in the ecological or evolutionary impacts of climate change
are encouraged to apply. We welcome applicants using any approach to
answer questions in

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New NSF mechanism for investigators at PUIs
Suzanne Lee
Thu, 7 Oct 2021 18:04:46 +0000
Reply
Dear ciliate colleagues,

In attending an NSF funding workshop today, I learned of an exciting new funding mechanism for new PIs at primarily undergraduate institutions (must be within 3 years of appointment by proposal submission date). The solicitation was just approved and will be updated soon, so worth keeping an eye on this page for more on eligibility: https://beta.nsf.gov/funding/opportunities/building-research-capacity-new-faculty-biology-brc-bio. Full proposals are due in Jan and June.

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Open postdoctoral position at the I2BC, France
Mireille BETERMIER
Mon, 4 Oct 2021 14:31:27 +0200
Reply
Dear colleagues,

A three-year postdoctoral position is open in my lab to work on
programmed genome rearrangements in Paramecium (https://bit.ly/3zEEwh7).

Would you please forward the attached announcement to anyone you think
may be interested?

Best regards,

Mireille Bétermier

--
Reply
No Replies
tenure track position (animal physiology)
Wei-Jen Chang
Sun, 5 Sep 2021 23:53:55 -0400
Reply
Dear colleagues, please help share the news to whoever may be interested in
our open position. Thanks.

The Biology Department at Hamilton College invites applications for a
tenure-track position in the area of animal physiology, at the rank of
Assistant Professor, beginning July 1, 2022. Ph.D. and teaching experience
required; post-doctoral research preferred. The teaching load for this
position is four courses during the first year and five courses thereafter.
The successful applicant will teach a lab course in vertebrate physiology,
a course in a related sub-discipline (e.g. immunology, endocrinology),
participate in our introductory biology program, and supervise senior
thesis

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Invitation to participate in 6th Annual International eConference of INSCR
renu gupta
Mon, 16 Aug 2021 16:00:17 +0000
Reply
Dear Prof./ Dr/ Researcher/ Student

Hope this mail finds you well!

We are very pleased to invite your esteemed institute to participate in the 6th Annual International eConference of International Network of Soil Contamination Research-INSCR 2021 on “MICROBES IN SUSTAINABLE DEVELOPMENT” being organized in association with Department of Zoology, University of Delhi , Acharya Narendra Dev College, Deen Dayal Upadhyaya College, Gargi College, Kirori Mal College, Maitreyi College, Ramjas College, Sri Venkateswara College, University of Delhi (DU), PG Department of Zoology, Magadh University, C.M.P. College, Allahabad University & Phixgen Pvt. Ltd, from 15th to 18th November 2021.

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Position Opening - WWU Mass Spec Technician Research Associate
Suzanne Lee
Tue, 10 Aug 2021 04:30:11 +0000
Reply
Dear colleagues,

Please share this with your networks and anyone who might be interested or know of someone who might be interested. Expertise in discovery proteomics and metabolomics would be especially welcome.

Many thanks for your attention and help!
Suzanne
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No Replies
Depression Slides for Paramecium Cultures
Simran Bhullar
Wed, 21 Jul 2021 13:11:21 +0000
Reply
Hello All,

I am writing to inquire about the 3-well depression slides which we normally use for paramecium cultures. Is anyone interested in buying some? I contacted the Polish company and they quoted a discount price of 6 Euros per slide and after taxes, it is coming out to be approximately 7.4 Euros per slide. This does not include shipping and handling to India, so I guess the cost will go upto 8.5 or may be 9 Euros per slide.

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Re: Depression Slides for Paramecium Cultures
Aufderheide, Karl J
Wed, 21 Jul 2021 20:30:58 +0000
Years ago, in the Sonneborn lab, he had the 9 hole slides taken to a glass shop to cut into 3 hole strips and smoothed off. If that service is available, that might be an option. Some of us in the Sonneborn clone still have boxes of the "old" culture slides in our collections. 

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contractile vacuole antibodies
Aaron Turkewitz
Tue, 20 Jul 2021 19:05:14 +0000
Reply
Hello ciliate colleagues
A member of my lab has become interested in studying the contractile vacuole in Tetrahymena. At this early point in the work, identifying some additional markers could be immensely useful. I’m wondering if any of you might have antibodies that strongly label the contractile vacuole, that you might be willing to share? (Of course if they’re commercially available, we can purchase them.)
Thanks very much
Aaron

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Protists in Barcelona
John Kloetzel
Thu, 15 Jul 2021 16:43:12 -0700
Reply
I would like to make the membership aware of an exhibition currently running at the Center for Contemporary Culture in Barcelona. Entitled “ScienceFriction”, it is designed in part to honor the contributions of our late colleague Lynn Margulis and her ideas on symbiosis in eukaryote evolution.
An Ashland (Oregon) artist, Shoshanah Dubiner, created a painting entitled “Endosymbiosis” on this subject, featuring a number of protists, fanciful and realistic (included in the pdf below). Shoshanah was asked to attend the memorial symposium for Lynn at UMass Amherst in March of 2012 with her painting.
It turns out that animators associated with

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2021 Ciliate Molecular Biology Meeting
Pearson, Chad
Tue, 6 Jul 2021 16:30:23 +0000
Reply
Hello all

We are pleased to announce that the schedule for the upcoming Ciliate Molecular Biology Meeting is posted on the website.
https://ciliates2020.wixsite.com/website

A Zoom link that will host both talks and synchronous poster sessions will be provided soon. Posters will also be available asynchronously through Confex and that information will also be provided by Confex soon.

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2021 Ciliate Molecular Biology Meeting (registration and abstracts due on June 15, 2021)
Jacek Gaertig
Wed, 9 Jun 2021 14:16:42 +0000
Reply
MEETING ANNOUNCEMENT:

Dear Ciliate Molecular Biology community:

The members of the Ciliate Advisory Board are pleased to announce the "2021 Ciliate Molecular Biology Meeting" to be held between July 19th-23rd, 2021. While we will continue the virtual format for 2021, we anticipate holding an in-person meeting in 2022. As was the case last year, this 2021 meeting will be free for all attendees!

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A postdoc position available
Jacek Gaertig
Mon, 7 Jun 2021 17:34:38 +0000
Reply
Dear Colleagues:

I would be grateful if you could forward the information about a new position to individuals who may be interested in joining our group.

A postdoctoral position is available in our laboratory at the University of Georgia to study cytoskeletal organelle biogenesis and intracellular pattern formation in Tetrahymena. The project will involve forward genetic approaches (suppressor screens), bioinformatics, genome editing and advanced microscopy.

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Director, Microscopy and Imaging Facility
Wei-Jen Chang
Sun, 30 May 2021 22:53:55 -0400
Reply
Dear colleagues,

The director of our microscopy and imaging facility is retiring and we are
looking for someone who knows how to maintain (ideally) both an SEM and
confocal microscope (detailed see below). I greatly appreciate if you can
help spread the words and we intend to start reviewing candidates in two
weeks.
Job link: https://apply.interfolio.com/88170

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2021 Ciliate Molecular Biology Meeting
Jacek Gaertig
Wed, 14 Apr 2021 15:05:00 +0000
Reply
MEETING ANNOUNCEMENT:

Dear Ciliate Molecular Biology community:

The members of the Ciliate Advisory Board are pleased to announce the "2021 Ciliate Molecular Biology Meeting" to be held between July 19th-23rd, 2021. While we will continue the virtual format for 2021, we anticipate holding an in-person meeting in 2022. As was the case last year, this 2021 meeting will be free for all attendees!

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Ian Gibson's death
Dilts, Judith
Sun, 11 Apr 2021 16:48:40 +0000
Reply
After a year-long battle against pancreatic cancer, Ian Gibson passed at home last friday. He is survived by his wife Liz and daughters Helen and Dominique. if you wish to send condolences, Liz's email is [log in to unmask]<mailto:[log in to unmask]>
below are some press releases on Ian's passing.
https://www.bbc.com/news/uk-england-norfolk-56695263
https://www.edp24.co.uk/news/tributes-to-former-norwich-north-mp-ian-gibson-7887274
https://www.itv.com/news/anglia/2021-04-10/former-norwich-north-mp-dr-ian-gibson-has-died
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One year visiting faculty position in developmental biology
Wei-Jen Chang
Tue, 16 Mar 2021 16:42:55 -0400
Reply
We are hiring a one-year visiting faculty because a colleague will be on
sabbatical leave. (https://apply.interfolio.com/85348)

Description

The Biology Department at Hamilton College invites applications for a
one-year position at the rank of Visiting Assistant Professor, beginning
July 1, 2021. We seek candidates to teach a lecture/lab course on
Developmental Biology, an introductory biology course, and two additional
course credits as complements to the department's offerings. These may
include: a lecture/lab course, a non-majors lecture course, an upper-level
seminar, or supervision of senior thesis research. We are seeking
candidates who can demonstrate their experience in teaching or working with
diverse

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I2BC team leader in cell biology call
Anne-Marie TASSIN
Tue, 16 Mar 2021 14:40:42 +0100
Reply
Dear all,

Our institute (Institute for Integrative Biology of the Cell- I2BC) is
seeking to recruit junior and mid-career group leaders in the field of
cell biology.

If you are interested, find below the announcement

https://www.nature.com/naturecareers/job/group-leaders-institute-of-integrative-biology-of-the-cell-i2bc-736371
<https://www.nature.com/naturecareers/job/group-leaders-institute-of-integrative-biology-of-the-cell-i2bc-736371>

Best regards

Anne-Marie Tassin

--
Anne-Marie Tassin
Institut de Biologie intégrative de la cellule
UMR 9198,
Avenue de la Terrasse
91198 Gif-sur-Yvette cedex,
France
Tel +33 (0)169823213
Fax +33 (0)169823181
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ciliate usernames
Bob Coyne
Mon, 15 Mar 2021 11:17:38 -0400
Reply
Dear Fellow Ciliate Nerds,
Today I set up an account with the US Social Security Administration and was asked to provide a username that does not include my name. Searching for an obscure, but easy to remember word, I chose Tetrahymena. I was surprised to learn that this username is already in use. Next, I tried thermophila. Also already in use. I won’t tell you how far down the nerd rabbit hole I had to go to find a unique ciliate username. I guess this says something about our community. Wishing you all the best.
Bob
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Tetrahymena vorax
Hennessey, Todd
Fri, 26 Feb 2021 17:15:40 +0000
Reply
Does anyone have some Tetrahymena vorax strain V2S that they could share with me?

Todd Hennessey
Dept. of Biological Sciences
H610 Hochstetter
University at Buffalo
Amherst NY 14260
716 645 4973
[log in to unmask]<mailto:[log in to unmask]>
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for the ciliate community: Positions in the Landweber lab at Columbia University
Landweber, Laura
Wed, 24 Feb 2021 23:07:52 +0000
Reply
Postdoctoral Positions and Research Staff: Columbia University in the City of New York
Genome Rearrangement, Transposon Recruitment, Epigenetics, Chromatin, and Non-coding RNA

Postdoctoral and staff positions are available in the Landweber lab in all areas of Oxytricha biology, including a joint position with the neighboring laboratory of Samuel Sternberg (sternberglab.org). The joint position will focus on transposase recruitment and domestication in programmed genome rearrangements in the ciliate Oxytricha, combining expertise from the Landweber lab in Oxytricha biology with expertise from the Sternberg lab in transposase recruitment for RNA-guided genome editing in CRISPR–Cas systems. We are seeking to hire a postdoctoral

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Coregulation Data Harvester news!
Lev Tsypin
Fri, 29 Jan 2021 20:23:46 -0800
Reply
Dear ciliate community,

It's been really rewarding to first develop the Coregulation Data Harvester
(CDH) in Aaron Turkewitz's lab in 2015 and to see more labs starting to use
it since our publication <https://doi.org/10.1016/j.softx.2017.06.006> in
2017. For those of you who are unfamiliar with the CDH, it's a program that
allows you to quickly gather functional information about coregulated genes
in *T. thermophila*, after identifying their orthologs in other organisms,
and thereby gain insight into likely functions of novel or simply
unannotated genes. We've and our collaborators have been able to
experimentally validate a lot of candidates that the program

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Mass Spectrometry Research Associate position
Suzanne Lee
Mon, 25 Jan 2021 07:09:25 +0000
Reply
Dear colleagues,

I apologize for the non-ciliate focused nature of this announcement, but my thought is that if there are qualified candidates that our community might know of who might interested and also have experience with proteomics using ciliates, it would be terrific to have them apply!

We recently got NSF MRI and Murdock Foundation funding to support the acquisition of new Mass Spec instrumentation and are now seeking a staff member whose role would be to facilitate the use of these equipment for research and teaching purposes at my university, Western Washington University in lovely Bellingham, WA. I would

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Lab Coordinator Position at Hamilton College
Wei-Jen Chang
Tue, 12 Jan 2021 14:48:00 -0500
Reply
Dear colleagues,

A long time lab coordinator in my department has just retired and we are
looking for their replacement. The link to the job description and
application is https://apply.interfolio.com/82792

Thanks and Happy New Year to everyone!

WJ
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A question
Ronald E Pearlman
Thu, 10 Dec 2020 16:10:33 +0000
Reply
Dear colleagues: I have been asked by the publishers MDPI to edit a special issue of their journal Microorganisms on the topic of Tetrahymena thermophila. If this is deemed to be of value to the community, I would be willing to do this. Before committing to possibly doing this, I would appreciate some feedback from the community. 

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Re: A question
Chalker, Douglas
Sat, 5 Dec 2020 15:13:14 +0000
Dear Ron,
I reached out to David Schultz at Seashell Technologies
I got the response below that said that they still have them and request a quote:
From: DAVID SCHULTZ <[log in to unmask]<mailto:[log in to unmask]>>
Hello Doug,

Thanks for reaching out to us. Yes, we are still supplying ballistic particles. If you would like a quote, please let us know the type and quantity that you want. Please note that the Minimum order quantity is $250.

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A question
Ronald E Pearlman
Sat, 5 Dec 2020 14:27:52 +0000
Reply
We have been purchasing gold particles for biolistic transformation from Seashell Technology Inc. Unless we are having internet problems accessing their www site, we are unable to reach them through their www site and the internet. Does anyone know if the company still exists or if someone else has taken over their business? Bottom line is does anyone have information about where to purchase gold beads at present? 

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Lea Bleyman
Peter J. Bruns
Wed, 18 Nov 2020 20:34:26 +0000
Reply
I was fortunate to overlap with Lea in Dave Nanney’s lab when she was a post doc and I was a grad student. I continued to see Lea after we both left Urbana, first in North Carolina and then, for many years, in New York. Lea’s characteristic vigor and zest for life were ever present. She was outspoken and always had worthwhile and fun things to say. She seemed most happy in New York and it was always a pleasure to visit her in that context. I will miss her larger than life presence.
Peter Bruns
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Obituary for Lea Kanner Bleyman
Lea Bleyman
Tue, 17 Nov 2020 22:35:16 +0000
Reply
For any questions about the below, please contact Anne Bleyman at [log in to unmask]

Obituary

Bleyman-Lea Kanner

Lea Kanner Bleyman died on November 6, 2020, three days short of her 84th birthday. Professor Emerita, Department of Natural Sciences, Baruch College; past-President International Society of Protozoologists (as it was then known). Lea was born in Halle, Germany, November 9, 1936. Escaping with her family to France in 1939, just before WWII, Lea spent time sheltered in OSE homes (for Jewish orphans) before a stint as a hidden child, living in a convent and on a farm to protect her from Nazi terror. Post-War,

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Re: Obituary for Lea Kanner Bleyman
F P Doerder
Wed, 18 Nov 2020 16:54:08 +0000
Sad news. Lea was a post-doc in Dave Nanney's lab when I joined it as a graduate student in the late 1960s. She was most helpful in initiating me into the wonders of ciliate genetics and an inspiring presence afterward at our ciliate meetings. She was a good story teller, especially stories about Tracy Sonneborn. My condolences to her family.
Re: Obituary for Lea Kanner Bleyman
Aufderheide, Karl J
Wed, 18 Nov 2020 18:43:18 +0000
I met Lea when I was doing a postdoctoral in Tracy Sonneborn's lab in the mid-1970s. She was so pleasant to share science with; I really enjoyed meeting her and becoming a friend.

Those years in Indiana in Sonneborn's lab were really special, and I often miss the people, the experiences and interactions that were possible there.

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Re: Obituary for Lea Kanner Bleyman
Lea Bleyman
Wed, 18 Nov 2020 19:41:49 +0000
Thank you all for these remembrances.

Do any of you know if Jean Nanney is still around and do you have contact info for her?

Anne Bleyman

[log in to unmask]
Re: Obituary for Lea Kanner Bleyman
John Kloetzel
Wed, 9 Dec 2020 17:50:54 -0800
Anne et al —
How well I remember Lea. She always seemed to me the epitome of “bright-eyed and bushy tailed”! Lea succeeded me at President of the Society of Protozoologists, and we worked together on the issues the Society was going thru during that time — we were making efforts to “internationalize” the Society, which has made some progress. It was always such a pleasure to encounter Lea and her beaming smile when arriving at professional meetings, and catching up. I can imagine that her students adored her.

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Protocol
Ronald E Pearlman
Fri, 23 Oct 2020 16:26:11 +0000
Reply
Does anyone have a protocol that they have used for Tetrahymena for ribosome/polysome profiling?

If so, could they please send me the protocol or provide a published reference where I can find this protocol.

Thank you very much. Any information/assistance will be very much appreciated

Ron Pearlman

Dr. Ronald E. Pearlman [log in to unmask]<mailto:[log in to unmask]>

Past President and Advisor

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Jytte Nilsson
kaczan
Sat, 26 Sep 2020 01:33:38 +0200
Reply
Dear Ciliatologists
I have just received the following E - mail from son of Jytte Nilsson
about Her passing away :

"It is with deep sorrow that I must inform you that Jytte has passed
away.
She was so exited and happy when you contacted her last year.
Kindly do not hesitate to contact me.
Best Regards , her son.
Steen Linderstrøm-Lang
Mail : [log in to unmask]
Phone: +4540441110 "

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Fw: Jytte Nilsson
Ronald E Pearlman
Sat, 26 Sep 2020 02:05:19 +0000
Very sad news about Jytte's passing. I came to Copenhagen to the Biological Institute of the Carlsberg Foundation led by Erik Zeuthen as a young postdoc on an adventure to live and work in Europe in a new environment. It was an active scientific environment that Jytte was a strong contributor to. Our areas of interest and expertise were quite different, she a cytologist and cell biologist and me a biochemist. I recall much good scientific interaction with Jytte. What I remember most was her as a lovely, warm, caring person who was very wonderful in helping me get oriented 

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N-terminal tagged plasmids
Ronald E Pearlman
Sun, 13 Sep 2020 13:57:21 +0000
Reply
We are looking for an N-terminal tagged plasmid for transformation into Tetrahymena and we would use this to insert our gene(s) of interest to transform Tetrahymena at the gene's endogenous locus. If an N-terminal FZZ tagged plasmid is available, that would be wonderful but any tag is fine.

Please let me know if such a plasmid is available and if you are willing to send it to us.

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No Replies
Janine Beisson
kaczan
Mon, 7 Sep 2020 08:48:19 +0200
Reply
Dear Linda and dear Jean,
We are shocked and very sorrowed by the news about tragic accident and
sudden death of Janine Beisson. We met Her many times at some occasions.
Long time ago Janina visited Janine Beisson Lab in Paris. Once Janine
and Her husband paid us a visit for a dinner at our appartment in Warsaw
during their short stay in Poland. It was a plesure and great honour for
us. The first thing which comes to our mind, when we think about Janine
is Her work on heredity of ciliary rows with reversed polarity in
Paramecium and

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Show Replies 11 Replies
Re: Janine Beisson
Ed Orias
Sat, 5 Sep 2020 10:18:18 -0700
Dear Jean and Linda,
My heart-felt condolences for the loss of Janine Beisson. I fondly
remember a visit to her and her highly creative and productive group in
l975, while on sabbatical leave in Copenhagen.
Sadly,
Eduardo Orias

On 9/5/2020 2:33 AM, Linda Sperling wrote:
>
> Dear colleagues,
>
> We are very sad to inform the ciliate molecular biology community that
> Janine Beisson passed away last Sunday 30 August, victim of an
> automobile accident while touring the French Alps. She was 89.
>
> After a long research career and important contributions to genetics,
> non-Mendelian

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Re: Janine Beisson
Peter J. Bruns
Sat, 5 Sep 2020 17:55:24 +0000
I too remember Janine fondly. She had a wonderful sense of life and a very bright intellect. What a loss.
Peter Bruns

Sent from my iPhone

On Sep 5, 2020, at 1:18 PM, Ed Orias <[log in to unmask]> wrote:



Dear Jean and Linda,
My heart-felt condolences for the loss of Janine Beisson. I fondly remember a visit to her and her highly creative and productive group in l975, while on sabbatical leave in Copenhagen.
Sadly,
Eduardo Orias

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Re: Janine Beisson
Mc Cutcheon, Suzanne M
Sat, 5 Sep 2020 17:58:33 +0000
Dear Linda and Jean,

The Buhse lab is very sad to hear of Janine Beisson passing. She was an inspiration to our group and a great colleague.

Condolences,
Suzanne McCutcheon

Suzanne M McCutcheon, Ph.D.

Clinical Assistant Professor

University of Illinois at Chicago

Department of Biological Sciences

840 W Taylor St, m/c067

Chicago, Illinois 60607
Re: Janine Beisson
Judith Van Houten
Sat, 5 Sep 2020 14:03:54 -0400
This is very sad news.  I am very sorry for your loss of Janine, and for
the loss to the ciliate community. Janine always was very helpful to me
and my group.
Judy Van Houten

Judith Van Houten, PhD
University Distinguished Professor Emerita

On 9/5/2020 1:18 PM, Ed Orias wrote:
>
> Dear Jean and Linda,
> My heart-felt condolences for the loss of Janine Beisson. I fondly
> remember a visit to her and her highly creative and productive group
> in l975, while on sabbatical leave in Copenhagen.
> Sadly,
> Eduardo Orias
>
> On 9/5/2020 2:33

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Fw: Janine Beisson
Ronald E Pearlman
Sat, 5 Sep 2020 19:39:55 +0000

Re: Janine Beisson
Aaron Turkewitz
Sat, 5 Sep 2020 20:01:25 +0000
Dear Jean and Linda,
I am truly sorry to hear this news. I first learned about Janine’s classic experiments on cortical inheritance in 1973, from an inspiring biology teacher named Pearl Strom. As I remember it, Strom spent an entire class period discussing Janine’s microsurgery experiments and what her findings might mean. This was during my first year of secondary school and the class had a huge impact one me – I know this because I remember very little else from that year! I had another intense encounter with Janine’s work twenty years later when I visited Chicago, to interview

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Re: Janine Beisson
Aufderheide, Karl J
Sat, 5 Sep 2020 20:50:13 +0000
This is very sad news indeed! Janine and I knew each other, and worked on some collaborative projects, since we met in Sonneborn’s lab in 1974.

We shall all miss her inspiration.

Karl J. Aufderheide, Ph.D.
Emeritus
Department of Biology
Texas A&M University
College Station TX 77843-3258

From: Ciliate Molecular Biology <[log in to unmask]> On Behalf Of Linda Sperling
Sent: Saturday, September 5, 2020 4:34 AM
To: [log in to unmask]
Subject: Janine Beisson

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Re: Janine Beisson
Pierangelo Luporini
Sun, 6 Sep 2020 20:31:51 +0200
Dear Linda and dear Jean,

really very sad to hear that Janine Besson passed away. I knew Janine, in
1975 in Gif-Sur-Yvette, as a pioneering organizer of an European meeting on
ciliate biology, and I will always remain fascinated by her unique capacity
to conjugate science with genuinely artistic illustrations of her beloved
paramecia. We miss a lovely person and a bright mind.
Piero

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Re: Janine Beisson
Helena Soares
Sun, 6 Sep 2020 21:52:44 +0000
Dear Linda and Jean,

It is sad to hear that Janine Besson passed away. I only meet her a few times but I was conquered by her ideas in several excellent papers that have changed my way to look at cells, especially the importance of positional information and intrinsic polarity in definition of specific territories inside of a single cell...I think that her work has a lot of clues and ideas that have not yet been completely explored !?

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Re: Janine Beisson
haga
Mon, 7 Sep 2020 17:19:18 +0900
Dear Jean and Linda,

 

This is very sad news.

I met Janine at the conference in the
suburbs of Paris in 2006.

She has performed many exciting works.

What I was especially impressed is the
demonstration of Sonneborn's insight into pattern

formation: A preexisting pattern
restricts a new pattern.

She has clearly demonstrated it by the
pattern formation in the cell surface structure of Paramecium.

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Re: Janine Beisson
Jacek Nowak
Mon, 7 Sep 2020 15:13:52 +0200
Dear Linda and Jean,

It is very sad to hear that Jannine passed away. We will remember her as a
person always willing to offer her help – both in the lab (isolate hundreds
of cells under a dissecting microscope, examine strange phenotypes) as well
as in life (give a lift, borrow a bike or a book). She was always ready to
share her scientific enthusiasm and deep knowledge of Paramecium biology.

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Janine Besson
Frankel, Joseph
Sun, 6 Sep 2020 19:25:20 +0000
Reply
One of Janine Beisson’s outstanding achievements was her success in gathering and maintaining (presumably also funding) a group of outstanding scientists, many but not all originally French, who were committed to analysis of the genetics and cell biology of a single genus, Paramecium (mostly Paramecium tetraurelia). In recent decades, Janine and her group greatly advanced our knowledge of cortical structures such as basal bodies and the infraciliary lattice, on the foundation of a detailed understanding of the proteins, such as tubulins and centrins, that make up these structures. 

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Janine Beisson
Linda Sperling
Sat, 5 Sep 2020 11:33:32 +0200
Reply
Dear colleagues,

We are very sad to inform the ciliate molecular biology community that
Janine Beisson passed away last Sunday 30 August, victim of an
automobile accident while touring the French Alps. She was 89.

After a long research career and important contributions to genetics,
non-Mendelian heredity, Paramecium cell biology and pattern formation,
Janine (almost) completely retired from the lab in 2018. Since then, she
had been in good health and was enjoying life.

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Tet gene annotation
Marcella Cervantes
Thu, 20 Aug 2020 16:10:23 -0400
Reply
Hello Everyone,
I hope you are well and enjoying the end of summer.
I have a question about gene annotation. What is the current best way to
identify introns and exons in Tetrahymena? I have tried the web version of
AUGUSTUS and NCBI ORF finder without much success. Has anyone trained
software to identify the exon/intron junctions and would be willing to
analyze two genes for me?
Any assistance would be greatly appreciated!
Marcella

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Show Replies 1 Reply
Re: Tet gene annotation
Marcella Cervantes
Thu, 20 Aug 2020 20:06:48 -0400
Hi Everyone,
I should clarify my question. I would like to predict the introns and exons
of the mating type I genes (MTA1, MTB1) in *T. thermophila *using software.
Mating type I is not included in the germline or somatic genome sequences
of *T. thermophila*. I only have the gene sequences, not the CDS or protein
sequences. Eventually, I will have the CDS from sequencing mRNAs.
Until then I was hoping to predict the CDSs to use for RNAseq analysis. Or
find out if someone has already sequenced the mRNAs for MTA1 and MTB1 and
would be willing to share

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Register for next week's 2020 Junior Investigator Ciliate Molecular Biology meeting (starts Monday!)
Sean Taverna
Thu, 16 Jul 2020 10:14:04 -0400
Reply
Dear Ciliate Community,

The *2020 Junior Investigator Ciliate Molecular Biology Meeting *is right
around the corner, and we have a great lineup of exciting presentations!
Support your colleagues (and meet new ones) and attend this virtual meeting
next week, from July 20-23rd, 10am-12:10pm (Eastern time zone).
*Although it is free to attend, you must first register so we can email you
the Zoom link and password.*

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Reminder - tell us soon if you want to present at the Junior Investigator Ciliate Molecular Biology Meeting
Sean Taverna
Wed, 24 Jun 2020 15:56:57 -0400
Reply
Dear Ciliate Molecular Biology community,

As a reminder, we would like to know your interest in presenting at the
upcoming Junior Investigator Ciliate Molecular Biology Meeting as soon as
possible (*preferably by this Friday June 26th*) to help us best organize
the sessions. The meeting will occur (virtually) over the week of July
20th-24th. Live (synchronous) presentations will be selected from
researchers at Undergraduate, Graduate, Postdoc and Assistant Professor
levels. Those not selected for live presentations will be given the
opportunity to submit a recorded presentation for viewing at any time by
meeting participants. The meeting will be open to

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Announcement- present at the 2020 Junior Investigator Ciliate Molecular Biology Meeting
Sean Taverna
Fri, 19 Jun 2020 14:21:27 -0400
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Dear Ciliate Molecular Biology community,

As many of you know, the pandemic necessitated the postponement of most
on-site academic meetings and conferences until at least next year.
Unfortunately, this timing means that our trainees will have fewer
opportunities to present their thesis research, and have reduced access to
essential feedback and recognition for their hard work. To address this
situation, members of TetRA board and the ciliate molecular biology
community are announcing a free, "*2020 Junior Investigator Ciliate
Molecular Biology Meeting" to be held virtually from July 20th-24th, 2020*.
The goal of this international conference is to highlight the research

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Laboratory Technician & Instructor in Biology at Hamilton College
Wei-Jen Chang
Fri, 12 Jun 2020 19:15:56 -0400
Reply
Application link: https://apply.interfolio.com/76571

Description

Primary responsibility is the preparation and teaching of laboratories for
upper level biology courses (200 level and above). This position works
full-time during the academic year, with 3 months off. Considered part time
with 75% schedule.

*Responsibilities:*

- Helps faculty develop and plan laboratories and create lab manuals.
- Prepares laboratories by gathering and setting out needed supplies and
equipment, preparing chemical solutions, tracking inventories, ordering
supplies, maintaining living material, etc. Also responsible for tearing
down laboratories and ensuring equipment is put away and materials are
cleaned.
- Teaches laboratories (3 hours each, 3 days/wk). This

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Revisions to Coregulation Data Harvester
Aaron Turkewitz
Mon, 11 May 2020 21:45:31 +0000
Reply
Dear ciliate colleagues

This note is with regard to the Coregulation Data Harvester, the online transcriptional profiling tool that some of you have used. The CDH was initially created by Lev Tsypin in my lab. This summer, I may have a computer science master’s student rewrite the code, with Lev (now a graduate student at Caltech) serving as advisor. My question to those of you who’ve used it: do you have a wishlist for additional features, or simply for some tweaks that would make the CDH more useful for you? If so, please send your ideas to me. Of course

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Open post-doc and PhD student positions at IBB, Warsaw, Poland
Jacek Nowak
Fri, 8 May 2020 16:43:31 +0200
Reply
Dear colleagues,

A 3-year post-doc and 4-year PhD student positions are open at Institute of
Biochemistry and Biophysics of the Polish Academy of Sciences, Warsaw,
Poland to study non-coding transcription in Paramecium.

Would you please forward the attached announcements to anyone you think may
be interested ?

Best,
Jacek K. Nowak, PhD
Reply
No Replies
How to Talk About the Corona Virus
Glenn Herrick
Wed, 1 Apr 2020 20:12:44 +0000
Reply
From below “How to Talk About the Coronavirus Four ways to help those around you be better informed about the pandemic”
https://www.theatlantic.com/ideas/archive/2020/03/how-talk-about-coronavirus/609118/

From my iPhone SE
Italy 39-349-056-0727
USA 01-801-448-5822
Please do NOT send email to my iCloud account, but to [log in to unmask] or [log in to unmask]

Begin forwarded message:

From: Nature Briefing <[log in to unmask]>
Date: April 1, 2020 at 20:11:11 GMT+2
To: Glenn Herrick <[log in to unmask]>
Subject: Evidence that cats (but not dogs) can be infected with the coronavirus
Reply-To: Nature Briefing <[log in to unmask]>

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POSTPONED: 2020 CILIATE BIOLOGY MEETING MOVED TO JULY 2021
Helena Soares
Wed, 18 Mar 2020 13:39:36 -0400
Reply
Dear Colleagues,

Out of an abundance of caution regarding the pandemic of COVID-19, the organizers of the 2020 CILIATE MOLECULAR BIOLOGY CONFERENCE have made the difficult decision to postpone the meeting, moving it to July 2021. The 2021 venue, speakers, and format are anticipated to remain the same, and the meeting will still occur in Lisboa, Portugal.

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Tetrahymena Stock Center activities temporarily suspended
Donna Cassidy-Hanley
Wed, 18 Mar 2020 12:34:34 -0400
Reply
As part of a campus wide response to the COVID-19 threat, the Tetrahymena Stock Center will temporarily suspend all shipments starting March 17, 2020 and continuing until the COVID-19 situation is resolved.

Check the website (https://tetrahymena.vet.cornell.edu/) for updates on when we will resume shipping.

Specific inquiries can be addressed to [log in to unmask] .

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cell morphology
Marcella Cervantes
Thu, 12 Mar 2020 11:06:11 -0400
Reply
Hi Everyone,
Does anyone know of a stain or method that will increase visualization of
cell morphology (cilia, oral apparatus, etc.) using a light microscope
rather than fluorescence?
Thank you for your help,
Marcella
Reply
No Replies
positions available
Aaron Turkewitz
Fri, 10 Jan 2020 15:19:48 +0000
Reply
My laboratory is starting a new project to analyze membrane trafficking pathways in Tetrahymena. The focus will include our long-term interest in mucocyst (secretory organelle) biogenesis but also phagosome formation and maturation. You can find examples of our recent work at:

https://www.sciencedirect.com/science/article/pii/S0960982218300800?via%3Dihub
https://www.biorxiv.org/content/10.1101/737247v1

Individuals interested in participating, either as postdocs or as research assistants (e.g., a 2-3 year position after college), are warmly encouraged to contact me for more information.
Thanks
Aaron

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2020 Ciliate Molecular Biology Conference-webpage
Helena Soares
Fri, 20 Dec 2019 11:33:04 -0500
Reply
SAVE THE DATE:
The 2020 Ciliate Molecular Biology symposium in Lisbon, Portugal will be held from July 14-18, at the Escola Superior de Tecnologia da Saúde de Lisboa (Lisbon School of Health Technology).

2020 Ciliate Molecular Biology Conference

July 14-18, 2020
Lisbon, Portugal
The conference website will be active soon.

Mark your calendars now to attend the 2020 Ciliate Molecular Biology Conference, July 14-18 in Lisbon, Portugal. The conference highlights recent research advances using ciliates, new tools and resources available for ciliate research, and novel community-wide initiatives in undergraduate research and education. At this meeting, interactions and discussions between scientists

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Plan to attend the 2020 Ciliate Molecular Biology Conference in Lisbon!
Sean Taverna
Wed, 27 Nov 2019 12:55:31 -0500
Reply
*SAVE THE DATE:*
The 2020 Ciliate Molecular Biology symposium in Lisbon, Portugal will be
held from July 14-18, at the Escola Superior de Tecnologia da Saúde de
Lisboa (Lisbon School of Health Technology).

*2020 Ciliate Molecular Biology Conference*

*July 14-18, 2020*
*Lisbon, Portugal*
*The conference website will be active soon.*

Mark your calendars now to attend the *2020 Ciliate Molecular Biology
Conference*, *July 14-18 in Lisbon, Portugal*. The conference highlights
recent research advances using ciliates, new tools and resources available
for ciliate research, and novel community-wide initiatives in undergraduate
research and education. At this meeting, interactions and discussions
between scientists

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PhD position: Programmed DNA Elimination
Francesco Catania
Fri, 22 Nov 2019 17:45:04 +0100
Reply
Dear colleagues,

The DFG funded Research Training Group "Evolutionary Processes in Adaptation and Disease" at the University of Münster, Germany, invites applications for 12 PhD Positions in biology, medicine, and philosophy (https://www.uni-muenster.de/EvoPAD/application/index.html <https://www.uni-muenster.de/EvoPAD/application/index.html> ).

One of these PhD positions is available in my research group. The successful candidate will focus on the process of Programmed DNA Elimination (PDE) in Paramecium. We are interested in understanding the impact of ecological changes on the fidelity of PDE and its consequences for genome evolution and adaptation.

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his-GFP or similar control protein?
Loren Hough
Thu, 21 Nov 2019 11:39:59 -0700
Reply
Hello all,

As a proof of principle experiment for NMR, we'd like to purify a highly
soluble, smallish (<40kDa) over-expressed affinity-tagged (his, GST, etc)
non-endogenous protein from tetrahymena. For those not familiar with our
work, we use the fact that tetrahymena readily eat bacteria to incorporate
heavy isotopes needed for biomolecular NMR. We've focused on tubulin, but
are hoping to see how generally applicable the system might be.

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Annual ASCB Lunch
Brian Bayless
Tue, 5 Nov 2019 12:16:32 -0500
Reply
Dear ASCB going ciliate community,

I hope everyone is doing well. ASCB is just around the corner and, as always, it would be nice to have a lunch to catch up and talk about all things ciliates. If you, or any of your students/postdocs, would like to attend please email me at: [log in to unmask] I would like to get a rough headcount so I can secure a lunch spot. Also, if you have a preference for Monday the 9th or Tuesday the 10th please let me know in your email.

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bad news
Angelici Maria Cristina
Thu, 31 Oct 2019 15:50:57 +0000
Reply
Dear Ciliate community

it is with great sorry that we have known the very sad news about the passing away of Marty Gorovsky.
On behalf of all the members ot the Italian Society, I send to you all, and especially to his family, our condolences.

M. Cristina Angelici
President of the Italian Society of Protistology (SIPonlus)
Reply
No Replies
On behalf of David Allis
Theodore G. Clark
Thu, 31 Oct 2019 01:44:06 +0000
Reply
Dear Ciliate Community Members,

I have written some of you some select emails as to my thought about Marty...this includes Joyce and his family, Jody, Jeff Hayes (who informed me of Marty's passing), Sean Taverna and a few other. Of course, I too echo all of what has been said about Marty, the scientist and Marty, the man. Both were off-scale.

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Marty Gorovsky
Theodore G. Clark
Tue, 29 Oct 2019 19:21:39 +0000
Reply
I will echo some of Eric’s remarks. One of my strongest memories from when I first joined the ciliate community was of Marty (along with Peter Bruns, Ed Orias, Liz Blackburn, Carol Greider, Joe Frankel) sitting in the front row at the ciliate molecular biology conference in Henniker, New Hampshire, ready to put his finger on the most important experiment someone hadn’t done. Although a bit intimidating, it was all done in good spirit and with the goal of getting to the scientific truth of the question at hand. Marty had an unrelenting critical eye but, like his companions in 

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Show Replies 18 Replies
Re: Marty Gorovsky
Suzanne Lee
Thu, 24 Oct 2019 04:31:43 +0000
Dear Jacek, Jody, Kathy and others,

I am so sad to hear this. I remember meeting Marty when I helped host him on a visit at Berkeley while I was a trainee in the Collins lab. On that visit, Marty asked probing scientific questions about my projects while also expressing enthusiasm and support. His support continued through generously sending strains, discussing scientific ideas over email, and writing letters to support my post doc fellowships. Just last year, Marty emailed me in response to a technical question I posed on this listserv, with helpful assistance and a big dose of encouragement

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Re: Marty Gorovsky
Chalker, Douglas
Thu, 24 Oct 2019 05:11:15 +0000
Dear Jody and Jacek,
Thank you for sharing this sad news. Marty was a role model for many of us. A outstanding, question-driven scientist and dedicated mentor to many. I’ll always remember him as a standard bearing for the ciliate molecular biology community. But more than that, he set a tone that continues to make our research community a special one. I remember once when he introduced me for a seminar at Rochester as a scientific grandson (Meng-Chao having been Marty’s first PhD student). I had never really thought about scientists as a “family” before that, but I can still

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Re: Marty Gorovsky
Aaron Turkewitz
Thu, 24 Oct 2019 07:46:01 +0000
I am so sorry to hear this sad news. No one was more supportive and encouraging for me than Marty, when I started working with Tetrahymena.
Such beautiful science, such human warmth.
Aaron

Sent from my iPhone

On Oct 24, 2019, at 6:35 AM, Suzanne Lee <[log in to unmask]<mailto:[log in to unmask]>> wrote:

Dear Jacek, Jody, Kathy and others,

I am so sad to hear this. I remember meeting Marty when I helped host him on a visit at Berkeley while I was a trainee in the Collins lab. On that visit, Marty asked probing scientific questions about my projects while also expressing enthusiasm and

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Re: Marty Gorovsky
Ed Orias
Thu, 24 Oct 2019 06:34:19 -0700
Dear all.
This is devastating news. Marty was one of the greatest molecular
biologists to take advantage of Tetrahymena to answer fundamental
scientific questions. While I did not have the privilege of being
directly mentored by him, I always benefited from his critical comments.
The Tetrahymena Genome Project would not have been gotten off the ground
without his essential contributions. My condolences to his family and to
the Ciliate community.
Eduardo Orias
Re: Marty Gorovsky
Judith Van Houten
Thu, 24 Oct 2019 10:46:54 -0400
We all have fond memories of Marty.
Judy

Judith Van Houten, PhD
University Distinguished Professor Emerita

On 10/24/2019 1:11 AM, Chalker, Douglas wrote:
> Dear Jody and Jacek,
> Thank you for sharing this sad news. Marty was a role model for many
> of us. A outstanding, question-driven scientist and dedicated mentor
> to many. I’ll always remember him as a standard bearing for the
> ciliate molecular biology community. But more than that, he set a tone
> that continues to make our research community a special one. I
> remember once when he introduced me for a

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Re: Marty Gorovsky
Eric Stephen Cole
Thu, 24 Oct 2019 10:24:00 -0500
As a grad student, and later as a postdoc,
the front row seats of every Ciliate meeting were occupied by
the village elders! Their ferocious attention to detail, combined
with their humor, generosity and gentleness, defined a "family" that
welcomed
all its newcomers. Marty was at the heart of this family, and
he left his mark on all of us. Grateful and sad, and happy to have known
Marty even a little.
Condolences (hugs) to all of you who were even closer to the heart of
our family, and must feel this passing even more acutely.
Eric

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Re: Marty Gorovsky
Pierangelo Luporini
Thu, 24 Oct 2019 19:33:28 +0200
Dear Jacek and Dear Ciliate Community,

very, very sad to hear that Martin passed away. Martin was co-organizer,
together with Peter Bruns, of the International Conference on Ciliate
Molecular Genetics at Cold Spring Harbor, in May of 1984. I always feel
extremely grateful to Martin for the invitation to attend this conference
(pioneer of the series of Gordon and Faseb meetings on ciliate molecular
biology), which was decisive for turning my major research interest on the
molecular basis of ciliate life.
My condolences to Martin's family.
Piero Luporini

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Re: Marty Gorovsky
F P Doerder
Thu, 24 Oct 2019 19:07:07 +0000
So sorry about this sad news. One of Marty's first papers inspired some of my early work, and of course, he had a very productive research and mentoring career that inspired so many others. He was always a pleasure to interact with at meetings, too. A loss to the community.
Paul Doerder
Re: Marty Gorovsky
Ronald Pearlman
Thu, 24 Oct 2019 16:32:53 -0400
I too am greatly saddened by this news. My interactions with Marty go back
a long time, to the late 1960s when I returned to Canada and started my
lab. With the late Jan Engberg, my first postdoc who I met when I was a
postdoc in Copenhagen and Jan was a graduate student, we were interested
in Tetrahymena rDNA. Meng Chao in Marty's lab was also interested in this.
We interacted very positively with the goal of advancing scince and this
was the first of many very interesting and productive interactions, many
leading to collaborations and co-publications.

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Re: Marty Gorovsky
Liu, Yifan
Fri, 25 Oct 2019 01:18:27 +0000
In his four decades of scientific career, Marty trained many international researchers, who often came to the US with stilted English, filled with passion for science, but never heard of Tetrahymena before. After a few years under Marty's wing, they will emerge as well-trained experimentalists, ready to make an independent career in the field of ciliate biology or beyond. Especially numerous among his trainees are Chinese researchers, with Meng-Chao as his first Ph.D. student. My wife (Yali) and I are lucky to be with Marty at the turn of the century, when the field of epigenetics, for which Marty was 

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Re: Marty Gorovsky
Yao, Meng-Chao
Fri, 25 Oct 2019 06:25:46 +0000
Dear Jody and Jacek:

I am devastated by this very sad news. Marty led the efforts to establish Tetrahymena as a model eukaryote for molecular studies, and trained a whole generation of researchers in this field including myself. He was my scientific father, and continued to be a supporter and guardian until I was able to stand on my own. We share so much together - I will cherish those memories in my remaining years. His passing marks the end of an era, and brought deep sadness to this scientific family.

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Re: Marty Gorovsky
<>
Fri, 25 Oct 2019 10:33:36 +0200
Dear Jacek,
The news about Martin Gorovsky passing away is really saddening. I met
him long time ago during my first post-doc,s in Iowa University and
Wesleyan University in 1968. I always have admired his discoveries on
tetrahymena nuclear apparatus.
I remember his lectures on many inetrnational conpherences; the whole
ciliate community will be missing important ciliate molecular
biologist.
With sorrow
Maria Jerka-Dziadosz

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Re: Marty Gorovsky
Klobutcher,Lawrence A.
Fri, 25 Oct 2019 14:08:09 +0000
This is indeed very sad news for the ciliate community. It is difficult to add to what has been said about Marty's role as a leader in the ciliate community, his scientific excellence, and his generosity in contributing to the scientific careers of many. I will add that the breadth of his scientific contributions is unusual, particularly in these times; he was involved in making significant findings in the fields of histone/chromatin biology, gene rearrangement, the cell cycle, and the cytoskeleton, as well as technical contributions to the field. I will miss him both scientifically and personally. 

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Re: Marty Gorovsky
Karrer, Kathleen
Fri, 25 Oct 2019 15:03:47 +0000
I am so sad to hear that Marty is no longer with us. He was a very good scientist and a very good man. The degree of rigor he brought to his science was something to aspire to. It seemed that his papers often had phrases like “we can’t eliminate the possibility”. It gave me the sense that he was considering all the possible, though perhaps unlikely, alternative explanations. I also appreciated his generosity to his mentees and colleagues. Although we all benefited from his incite and ideas at meetings, we didn’t often hear him give a presentation because he 

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Re: Marty Gorovsky
Peter Satir
Fri, 25 Oct 2019 19:12:32 +0000
We are sorry to learn of Marty Gororsky's death. We knew him when he was a graduate student at Chicago and first became interested in Tetrahymena. He was a major contributor to the field, keeping ciliate molecular biology alive and advancing. He will be missed.

Birgit and Peter Satir

From: Ciliate Molecular Biology [mailto:[log in to unmask]] On Behalf Of Jacek Gaertig
Sent: Wednesday, October 23, 2019 10:54 PM
To: [log in to unmask]
Subject: Marty Gorovsky

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Re: Marty Gorovsky
Shuguang Zhang
Fri, 25 Oct 2019 21:09:04 +0000
Dear Ed,

I only met Marty Gorovsky a few times at Ciliate meetings including the one at Pingree Park in 1983 and one at Cold Sprint Harbor in 1984. Although I was then only a fresh graduate student without speaking good English, I found him easy accessible and friendly to talk with us. When I asked him a question about ciliate transformation, it was then one of the bottleneck for ciliate molecular biology, he was very nice and thoughtful. I remember that when I was a graduate student in your lab, you frequently talk about not only his rigorous scientific

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Re: Marty Gorovsky
kaczan
Sat, 26 Oct 2019 09:43:42 +0200
Dear friends

I am very suddened by the news about passing away of
Martin Gorovsky. However I did not know him closely, I remember him as a
leader of the community of the Tetrahymena people, tireless promotor of
the Tetrahymena research and beyond this as friendly supportive and open
person. He was concerned very much with bringing Tetrahymena research to
the main stream biology.

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Re: Marty Gorovsky
C. David Allis
Wed, 30 Oct 2019 12:03:19 +0000
Dear Ted and ciliate community members,

I have written some of you some select emails as to my thought about Marty...this includes Joyce and his family, Jody, Jeff Hayes (who informed me of Marty's passing), Sean Taverna and a few other. Of course, I too echo all of what has been said about Marty, the scientist and Marty, the man. Both were off-scale.

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Marty Gorovsky
Peter J. Bruns
Mon, 28 Oct 2019 21:37:25 +0000
Reply
Dear ciliate colleagues, I was out of touch electronically when the word came out of the loss of Marty Gorovsky; I write to add to the many thoughts of the community. Marty was an immensely important and influential colleague and scientist. He contributed to the science of Tetrahymena specifically, and cell biology more generally. Focusing on gene regulation, he made major contributions throughout his long and productive career. Beyond that, he was an incredible mentor and colleague. So many of us were aided and supported by his generosity, his wisdom and his critical thinking, no matter if we were student 

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Marty Gorovsky
Jacek Gaertig
Thu, 24 Oct 2019 02:53:54 +0000
Reply
Dear Ciliate Researchers:

I am forwarding very sad news from Jody Bowen about the passing away of Marty Gorovsky. His studies led to many fundamental contributions in cell and molecular biology based on ciliate models, including groundbreaking discoveries on chromatin and genome organization. I had an honor and privilege to be among his many trainees. Marty was a wonderful mentor. It is a huge loss to our community.

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Job ad: Full or Associate Professor in Ecology
Zufall, Rebecca A
Wed, 23 Oct 2019 20:21:09 +0000
Reply
Dear colleagues,

My department at the University of Houston is searching for an Ecologist (broadly defined) at the Associate or Full Professor level (also able to consider Asst. Professors with sufficient experience to be hired with tenure).

Please pass this along to anyone who might be interested:
https://uhs.taleo.net/careersection/ex2_uhf/jobdetail.ftl?job=FAC001168

-Becky Zufall

Full or Associate Professor - Ecology - (FAC001168)
Organization
: H0104 Biology
Salary Commensurate with experience
Description
:

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invitation to a special issue in Microorganisms (MDPI)
Cristina Miceli
Wed, 18 Sep 2019 14:47:33 +0200
Reply
Dear Ciliate Biologists,

The journal Microorganisms (http://www.mdpi.com/journal/microorganisms, IF
4.167) is preparing a Special Issue entitled "Ciliate: From Genomics to
Signalling Systems", for which we are the Guest Editors.

This Special Issue is open to all approaches and studies seeking to
understand the complex mechanisms and evolution of signalling systems in
ciliates, from the involved genes to the changes in gene expression during
cell response, and from the structure and evolution of signal molecules to
the membrane traffic in cells and changes in cell structure and
organization.

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Bahamas relief (delete if not your cup of tea)
Eric Stephen Cole
Thu, 5 Sep 2019 15:33:21 -0500
Reply
Having some deep attachment to these islands and
the islanders I contacted the Director of the Cape Eleuthera
Institute and asked about a good place to offer financial support
for the residents of Grand Bahamas and Abaco. here is what he sent me:

"There is a grass roots Bahamian organisation called HeadKnowles that is
coordinating relief efforts for a number of Bahamian NGOs. They have
planes standing by ready to fly and are coordinating supplies collection in
Nassau - their biggest need is money to pay for aviation fuel. They have
a GoFundMe <https://www.gofundme.com/f/headknowles-emergency-funds> page
and I would recommend directing

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Visiting Assistant Professor of Biology (Molecular Geneticist)
Wei-Jen Chang
Tue, 27 Aug 2019 21:42:05 -0400
Reply
Dear colleagues,

Please help pass to interested colleagues and friends.
Description

The Biology Department at Hamilton College invites applications for an
eighteen-month position at the rank of Visiting Assistant Professor,
beginning January 1, 2020. Candidates with a completed Ph.D. and teaching
experience preferred. The teaching load for this position is the
equivalent of three courses during the first semester and five courses
thereafter. The successful applicant will teach a lecture/lab course on
Genetics (two course credits), an introductory biology course, and two
additional course credits as complements to the department's offerings.
These may include: a lecture/lab course, a non-majors lecture

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GRL locus overexpression plasmid
Dorota Wloga
Tue, 27 Aug 2019 11:52:31 +0200
Reply
Dear Ciliate Researcher,

Some years ago I came across a plasmid that enables overexpression of
Tetrahymena proteins under the control of MTT1 promoter in GRL3/GRL4
loci. We designed a similar plasmid for overexpression in these loci.
Does anyone know who first made this plasmid and which paper should be
cited or who should be acknowledged?
Many thanks in advance!

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Lab Reagents to Donate
JOHN KLOETZEL
Mon, 10 Jun 2019 16:22:17 -0700
Reply
Greetings, ciliate colleagues —
I have a problem: after retiring from Maryland to Oregon, I find that the bulk of the equipment and supplies I brought with me (to continue my research in my ‘home lab’ ... as I supposed … ) is going unused. Rather than sit on my shelves, I’d like to see these reagents used by someone working with protists. I’ve found homes locally for the more common supplies and equipment from my larder (Ashland High School, and Southern Oregon University). But some of the more specialized things typically are used only by folks like us, doing

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Election Results
Aaron Turkewitz
Wed, 5 Jun 2019 13:57:30 +0000
Reply
Dear Ciliate colleagues
The voting for new Tetra Board members is complete, with a total of 158 votes cast. The new Tetra board members will be:

Mireille Betermier
Marcella Cervantes
Chad Pearson

Thanks to all of you who participated, and especially to the candidates who accepted nomination and the opportunity to strengthen our community.

All the best
Aaron

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Looking for Tetrahymena CURE research partner
Carolyn Wetzel
Wed, 29 May 2019 15:17:37 -0400
Reply
I teach biology at a 2-year HSI community college in Western Massachusetts
and am setting up a new 200-level cell biology course to begin Fall 2020. I
am developing course materials for the lab, in which I would like the
students to run research projects using *Tetrahymena*.

The focus of the course is on cell biology, not molecular genetics, so I am
looking for possible partner projects that require the (~15-20) students to
analyze cell biology/physiology of mutants or tagged lines that someone
else has generated. Do you have a backlog of such lines that you want help
screening?

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TETRA Board elections
Aaron Turkewitz
Tue, 21 May 2019 17:42:13 +0000
Reply
In case it’s useful, here’s a message again that I’d sent previously, on the current composition of the TETRA Board.

Aaron

Dear colleagues-in-ciliates,

I am writing to inform you of an upcoming election to fill three vacancies in the Tetrahymena Research Advisory (TetRA) Board, and to invite you to submit nominations for candidates.

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Vote in TETRA BOARD elections
Aaron Turkewitz
Tue, 21 May 2019 17:11:30 +0000
Reply
To all members of the ciliate community,

Three positions on the 6-member TETRA Advisory Board are coming vacant. Four people have accepted nominations to run for these positions, and on behalf of the current board I would like to thank them.

You will find their names and position statements below. You will be receiving, by e-mail, instructions on how to vote in this election. The voting will be open until June 3rd.

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Open post-doctoral position at the I2BC, France
Mireille Betermier
Fri, 19 Apr 2019 13:16:37 +0200
Reply
Dear colleagues,

A 2-year post-doctoral postition is open in Gif-sur-Yvette to work on
Paramecium domesticated transposases and their partners in programmed
genome rearrangements
(https://emploi.cnrs.fr/Offres/CDD/UMR9198-MIRBET-001/Default.aspx?lang=EN<http://bit.ly/2PihOFW>).

Would you please forward the attached announcement to anyone you think
may be interested ?

Best regards,

Mireille Bétermier
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"Why Ciliates"
Theodore G. Clark
Tue, 2 Apr 2019 19:34:51 +0000
Reply
Dear All,

Thanks Aaron for referencing the “Why Ciliates” video on Vimeo in your previous email to the ciliate community. I would only point out that the correct link to that video is as follows: https://vimeo.com/191812936

Best, Ted
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Call for nominations for upcoming TetRA Board elections
Aaron Turkewitz
Mon, 1 Apr 2019 14:49:08 +0000
Reply
Dear colleagues-in-ciliates,

I am writing to inform you of an upcoming election to fill three vacancies in the Tetrahymena Research Advisory (TetRA) Board, and to invite you to submit nominations for candidates.

Background:

TetRA Board holds ~monthly meetings via Zoom to identify and act on issues that impact the ciliate molecular biology research community. Since its inception in 2011, TetRA Board has addressed a wide range of topics. They include (1) the security and expansion of community resources in stock centers and genome databases, (2) the challenges associated with re-tooling research in the era of genomics and bioinformatics, and effectively

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Special issue - Deadline extended
Martin Simon
Wed, 27 Mar 2019 14:59:41 -0400
Reply
Dear colleagues,

We are happy to announce that we extended the deadline for contributions of our upccoming Special Issue entitled “Ciliate Genetics and Epigenetics” launched by the journal Genes (ISSN 2073-4425; IF 3.2).

New deadline for manuscript submissions: 31 May 2019

This Special Issue provides an overview on the genetics and epigenetics in ciliates and their impacts on adaptation and evolution. We invite submissions for reviews, research articles, or short-communications reporting molecular genetics and epigenomics studies of ciliate research.

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this is not spam!
Judith Van Houten
Thu, 21 Feb 2019 16:22:29 -0500
Reply
Dear Ciliate researchers!Please read – not a predator journal scam!!

We are hoping to make ciliate research more visible and appreciated.

In collaboration with the journal *Frontiers in Cell and Developmental
Biology* <https://www.frontiersin.org/journals/403>, we are bringing
together a selected group of international experts to contribute to an
open-access article collection on:

*What Do We Learn About Cilia From Ciliate Model Systems? The Insights
From Cutting-Edge Approaches*
<https://www.frontiersin.org/research-topics/9254>

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advice on Coulter Counter
Aaron Turkewitz
Tue, 5 Feb 2019 13:27:03 +0000
Reply
We need to replace our Coulter Counter, which is the model in which you can set dual threshholds (the Z1-D). From what I can tell, the model with a single threshold (Z1-S) should be equally good for counting Tetrahymena, but does anyone have any experience with this?
Thanks very much
Aaron Turkewitz
Reply
Show Replies 1 Reply
Re: advice on Coulter Counter
Ronald Pearlman
Tue, 5 Feb 2019 12:43:03 -0500
Aaron: Our Coulter Counter died a number of years ago. It was too
expensive to fix or purchase a new one. For our very basic needs, we now
just use haemocytometer counting. That may not work for you but it has
satisfied our needs. It has been a while but my recollection is that the
single threshold Coulder Counter worked for us.

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Reminder: York Science Fellows (deadline - January 4, 2019) (fwd)
Ronald Pearlman
Mon, 3 Dec 2018 15:19:56 -0500
Reply
Please see below for the announcement of a lucrative 2 year postdoctoral
fellowship at York University that can be held in my lab in the Department
of Biology. I am willing to support an applicant for this award.

This award will be very competitive and there are unfortunately few awards
spread over the entire Faculty of Science. An interested applicant will
need to have a strong academic and publication record. The successful
applicant will need to have completed their Ph.D. and take up the award by
October 2019.

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ASCB lunch
Brian Bayless
Tue, 27 Nov 2018 17:04:03 -0500
Reply
Hi ASCB going ciliate community,

ASCB is right around the corner. The ciliate lunch will be held on Tuesday the 11th. If you have not RSVPed yet, please send me an email at [log in to unmask] It is open to all researchers interested in ciliate biology, including graduate students and postdocs. If you know of anyone who may be interested please forward them this email. Thank you and I look forwards to seeing some of you in sunny San Diego next week!

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automated image analysis of Tetrahymena cell shape?
Suzanne Lee
Tue, 27 Nov 2018 19:08:39 +0000
Reply
Dear Ciliate Communities,

I’m interested in doing some automated cell image analysis to measure cell shape. Has anyone done something similar? I’m envisioning a cytosolic stain or membrane marker that would allow for clearly defining the edges of a cell in order for image analysis software to detect and quantify cell dimensions.

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ASCB Ciliate Lunch
Brian Bayless
Mon, 29 Oct 2018 12:25:19 -0400
Reply
Dear ASCB going ciliate community,

I hope everyone is doing well. ASCB is just around the corner and, as always, it would be nice to have a lunch to catch up and talk about all things ciliates. If you, or any of your students/postdocs, would like to attend please email me at: [log in to unmask] I would like to get a rough headcount so I can secure a lunch spot. I promise to avoid restaurants with exceptionally loud Sitar players like we had last year. Also, if you have a preference for Sunday, Monday or Tuesday please let me know in your

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Special issue "Ciliate Genetics and Epigenetics"
Martin Simon
Wed, 24 Oct 2018 07:19:25 -0400
Reply
Dear colleagues,

This announcement serves to remind authors and readers of our upccoming Special Issue entitled “Ciliate Genetics and Epigenetics” launched by the journal Genes (ISSN 2073-4425; IF 3.600).

This Special Issue provides an overview on the genetics and epigenetics in ciliates and their impacts on adaptation and evolution. We invite submissions for reviews, research articles, or short-communications reporting molecular genetics and epigenomics studies of ciliate research.

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Positions available in Landweber lab, New York City (Columbia University)
Landweber, Laura
Wed, 8 Aug 2018 22:34:27 +0000
Reply
Dear all,

I have two positions available in my lab, a postdoc position, and a lab manager/staff associate position.

Please see the job postings at

https://www.rnasociety.org/jobs/postdoctoral-position-study-rna-guided-genome-rearrangements-columbia-university-new-york/
for the postdoc position

and

https://academicjobs.columbia.edu/applicants/Central?quickFind=66826 <https://academicjobs.columbia.edu/applicants/Central?quickFind=66826> for the staff associate/technician/lab manager position.

The full text of the postdoc ad is as follows:

RNA-Guided Genome Rearrangements at Columbia University (Landweber lab) in the City of New York

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plasmid request
Jacek Gaertig
Mon, 23 Jul 2018 17:45:54 +0000
Reply
Hi All,

Does anyone have a plasmid for targeting into the MTT1 locus with mCherry or any non-green fluorescent protein? For example a desired plasmid would have the following elements: MTT1-5’-neo5-MTT1-5’(promoter)-mCherry-myORF-MTT1-3’ ? Thank you in advance for your help!

Best Wishes

Jacek

Jacek Gaertig
Department of Cellular Biology
University of Georgia
724 Biological Sciences Building
Athens, GA 30602-2607
Phone: 706-542-3409
Fax: 706-542-4271
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GSA highlighting former ciliate research trainee
Doug Chalker
Fri, 20 Jul 2018 14:33:38 -0400
Reply
Dear Ciliate community,
Greetings from the Ciliate Molecular Biology Conference. You may want to read the interview with Sujal Phadke on maintaining work-life balance as an early career researcher.
http://genestogenomes.org/sujal-phadke-on-finding-work-life-balance-in-non-profit-research/

Here former PhD advisor, Becky Zufall, was one of three researchers on a discussion panel at the conference
a quote from the article.

"Becky Zufall, showed me what a good mentor should be. She supported my interests by allowing me to be independent and have the freedom to determine where to take my research."

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Re: GSA highlighting former ciliate research trainee
Ronald Pearlman
Fri, 20 Jul 2018 15:00:22 -0400
Doug: Apologies for not getting this sent earlier.

I don't know if you have already had the program honoring Denis Lynn that
was mentioned earlier. Whether or not that has occurred, I thought that
you and the community would like to be aware that the Canadian
Broadcasting Corporation (CBC), Canada's national public broadcaster, had
a segment about Denis last week on its flagship public affairs program As
it Happens. See
http://www.cbc.ca/radio/asithappens/ubc-professor-killed-by-rogue-wave-remembered-as-a-wonderful-person-1.4731053.
for a transcript of the session that contains a link to a 6 1/2' interview
about Denis with a former trainee.

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AW: Tetrahymena culture contamination
Josef Loidl
Thu, 19 Jul 2018 18:03:05 +0200
Reply
Dear Erhan,

We often had a similar problem. While Normocin does not help, Normocure (from the same company) does. We use it 1:500 in small cultures; after 2-3 days, the cells are usually cured.

Best regards,

Josef Loidl

Von: Ciliate Molecular Biology [mailto:[log in to unmask]] Im Auftrag von erhan aslan
Gesendet: Donnerstag, 19. Juli 2018 17:47
An: [log in to unmask]
Betreff: Tetrahymena culture contamination

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Tetrahymena culture contamination
erhan aslan
Thu, 19 Jul 2018 18:47:10 +0300
Reply
Dear Ciliate Community,

I am struggling with contamination in my *Tetrahymena* cultures for a
while. Routinely used antibiotics (pen-strep, amphotericin) don't work. I
used normocin from Invitrogen in conbination with pen-strep or kanamycin to
get rid of them but no success again. Contaminated wells appear whitish and
cloudy (Please see attachment). I noticed that this contamination generally
occurs after biolistic bombardment. Sterile cabinet was tested for the
possible contamination source but it seems OK. I extensively sterilize the
inner chamber of the biolistic gun (which is in our sterile cabinet) and
other components with alcohol prior to shot but they

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Re: Tetrahymena culture contamination
Daniela Sparvoli
Thu, 19 Jul 2018 16:34:53 +0000
Dear Erhan,

we use a very powerful mix of antibiotics to treat bacterial contamination in our lab, please see the attached protocol, and it works very well to clear Tetrahymena cultures.

I hope this helps.

Best regards,

Daniela.
Tetrahymena Comparative Genomics Database (TCGD) is online (http://ciliate.ihb.ac.cn/)
miaowei
Wed, 18 Jul 2018 19:29:27 +0800
Reply
Dear ciliate colleagues,

It’s my great pleasure to announce that the Tetrahymena Comparative Genomics Database (TCGD) is online (http://ciliate.ihb.ac.cn/).

TCGD now contain the genome sequences, transcriptome data, predicted genes, functional annotation, ortholog groups of newly sequenced nine Tetrahymena species. Among them, three species (T. borealis, T. elliotti, T. malaccensis) were sequenced by Broad Institute and JCVI (supported by NSF funding to Robert Coyne), and previously available in TGD wiki. TCGD have hyperlinks to direct the user to these three genomes in TGD wiki. The rest six genomes (T. pyriformis, T. vorax, T. canadensis, T. empidokyrea, T. shanghaiensis and T. paravorax)

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Tetrahymena genetics starter kit available
Bob Coyne
Mon, 16 Jul 2018 20:39:28 -0400
Reply
I have some lab equipment that I'd like to pass on to some worthy scientist doing ciliate genetics. They are:

1. Two 96-well replica plating tools (thin and thick prongs)
2. One 48-prong "drop maker" used for, well, making drops (obviously) in a petri dish for isolating single cells or mating pairs. If you've seen one, you know what I mean. As far as I know, they are only used for Tetrahymena, but maybe they would be useful for other ciliates.
3. Two 48-prong replica tools for replicating the above drop plates (thin and thick prongs).
4. One 12-channel Costar

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Postdoc in the Winey lab...
Mark Winey
Mon, 16 Jul 2018 13:36:29 +0000
Reply
Hello all -

The Winey lab at UC Davis is accepting applications for a postdoctoral position to do biochemistry on Tetrahymena axonemal proteins.

This position is funded with good benefits, and here is the link to apply:

https://recruit.ucdavis.edu/apply/JPF02298

Let me know if you have questions.

Thanks,

Mark Winey

[log in to unmask]
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Reminder:Introducing short talks at the 2018 Ciliate Molecular Biology Conference
Doug Chalker
Fri, 13 Jul 2018 11:36:47 -0400
Reply
Dear Fellow Ciliate Molecular Biology Conference Participants

Reminder:
We have a new opportunity to present your work at the conference – “Lightning talks”. What is a lightning talk? In short you have 2 minutes to make your pitch. We still have room for a few more
Need more inspiration?
Did you have a poster and want to describe your coolest result on stage?
Did you develop a new technique that you want to share?
Did you come up with the next great idea of what to study and just need to pitch it?
Do you have a need to rap

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Survey regarding attendance at the 2020 Ciliate Conference
Doug Chalker
Thu, 12 Jul 2018 15:23:51 -0400
Reply
Dear Ciliate Molecular Biology Community

As community members at the 2018 Ciliate Molecular Biology Conference will vote on where to hold the 2020 meeting, we would like to give everyone an opportunity to indicate factors that would make their attendance 2020 Ciliate Molecular Biology meeting more or less likely. Please respond to the easy 10 question survey linked below.
Thank you

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Denis Lynn
Christian F. Bardele
Sun, 1 Jul 2018 16:16:35 +0200
Reply
What a sad news. Drowning while collecting his small little friends, the ciliates, which made him such an outstanding scientist, is an unbelievable irony of fate, which makes me almost speechless. On June 19, June we communicated via email on another joint project. Denis told me of his soon trip to Haikai Institute on Calwert Island and informed me, that he would send me a missing drawing immediately after his return. Now, all is gone – I lost a dear friend and an excellent colleague - I’m so sad. 

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Re: Denis Lynn
Elzbieta Wyroba
Thu, 28 Jun 2018 16:03:38 +0200
_A GREAT LOSS TO OUR COMMUNITY..._

_ELZBIETA WYROBA_

_ _

> I'm very sorry to share the sad news I've just received, that Denis Lynn died yesterday while conducting field work on the central coast of British Columbia.
>
> Aaron Turkewitz
Re: Denis Lynn
Judith Van Houten
Thu, 28 Jun 2018 10:39:07 -0400
He will be missed.
Judy

Judith Van Houten, PhD
University Distinguished Professor Emerita

On 6/28/2018 6:48 AM, Aaron Turkewitz wrote:
>
> I'm very sorry to share the sad news I've just received, that Denis
> Lynn died yesterday while conducting field work on the central coast
> of British Columbia.
>
>
> Aaron Turkewitz
>
>>>>
Re: Denis Lynn
Asai, David J
Thu, 28 Jun 2018 15:08:05 +0000
Thank you, Aaron, for sharing the news.
Denis was a scholar and a leader, and his contributions will persist. A loss much too early.
David

David J. Asai
Senior Director, Science Education
Howard Hughes Medical Institute
301-215-8874
[log in to unmask]<mailto:[log in to unmask]>
http://www.hhmi.org/developing-scientists

From: Ciliate Molecular Biology <[log in to unmask]> On Behalf Of Aaron Turkewitz
Sent: Thursday, June 28, 2018 6:48 AM
To: [log in to unmask]
Subject: Denis Lynn

I'm very sorry to share the sad news I've just received, that Denis Lynn died yesterday while conducting field work on the central coast of British Columbia.

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Re: Denis Lynn
F P Doerder
Thu, 28 Jun 2018 15:51:59 +0000
Stunning, sad, news. Denis' encyclopedic knowledge of ciliates, ranging from the morphological to the molecular to the ecological, was unparalleled. He was a long-time friend and wonderful collaborator. Denis will be missed. Paul Doerder
Re: Denis Lynn
Aufderheide, Karl J
Thu, 28 Jun 2018 15:55:14 +0000
Very sad news. Dennis was a fine colleague, always eager to share knowledge and ideas.

Karl J. Aufderheide
Associate Professor
Department of Biology
Texas A&M University
College Station TX 77843-3258
979-845-7775

From: Ciliate Molecular Biology [mailto:[log in to unmask]] On Behalf Of Aaron Turkewitz
Sent: Thursday, June 28, 2018 5:48 AM
To: [log in to unmask]
Subject: Denis Lynn

I'm very sorry to share the sad news I've just received, that Denis Lynn died yesterday while conducting field work on the central coast of British Columbia.

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Re: Denis Lynn
Gregory A Antipa
Thu, 28 Jun 2018 16:28:23 +0000
Sad unexpected times for All,

greg

Gregory Antipa
Professor Emeritus
Department of Biology
San Francisco State University
San Francisco CA 94132

On Jun 28, 2018, at 8:55 AM, Aufderheide, Karl J <[log in to unmask]<mailto:[log in to unmask]>> wrote:

Very sad news. Dennis was a fine colleague, always eager to share knowledge and ideas.

Karl J. Aufderheide
Associate Professor
Department of Biology
Texas A&M University
College Station TX 77843-3258
979-845-7775

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Re: Denis Lynn
Ronald Pearlman
Thu, 28 Jun 2018 12:36:04 -0400
Aaron: Very sad news and thanks for sharing.

Denis will certainly be missed but his many contributions will live on
with his published work and his many other contributions and
collaborations which will always be cherished by colleagues.

Our paths crossed moderately frequently when he was at Guelph which is
only an hour from Toronto where I am. But as always, we saw each other and
interacted much too infrequently and that became even less frequent when
he moved west.

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Re: Denis Lynn
H Long
Sat, 30 Jun 2018 00:03:36 +0800
I was astonished to hear this aweful news. My first new ciliate species
(Frontonia lynni 2015)was dedicated to him and got advised by him for one
hour in ciliatology at Weibo Song's lab as a masters student.

He will be deeply missed by our Chinese ciliate research community.

Hongan

On Friday, June 29, 2018, Juan Carlos Gutierrez <[log in to unmask]> wrote:

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Re: Denis Lynn
Eric Stephen Cole
Sat, 30 Jun 2018 09:10:31 -0500
It' really hard to respond to this news. My wife, Kate and I visited
Denis in Guelph when I was just starting out after grad school.
(We wondered about the possibility of my teaching at a school
like his). Denis was a sweet and doting host. Over the years,
I had so many good interactions through the journal, and at
meetings I was always genuinely excited to run into him again. Denis
brought such clarity and breadth to the truly daunting task of
making sense of protozoan taxonomy, at a time when we really
needed clear leadership in that business.

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Re: Denis Lynn
Pierangelo Luporini
Sat, 30 Jun 2018 19:49:43 +0200
I wish to join the ciliatologist community in this very sad moment of Denis
Lynn loss. I had the pleasure to spend smiling days together with Denis and
Portia here in Italy, visiting the surroundings of Camerino, Versilia and
Le 5 Terre. I enjoyed and deeply appreciated His talking of science, His
philosophy of life, His extremely friendly behavior. Certainly, as Ed
writes, "If there is any consolation, he was still in the act of doing what
he had loved to do all his life". Nonetheless, it is really hard to accept
that a cause to which a Life was

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FW: Denis Lynn
Aaron Turkewitz
Sun, 1 Jul 2018 19:44:24 +0000
Sharing this message I received from Andrzej Kaczanowski, since I think it belongs to the community.

From: kaczan <[log in to unmask]>
Date: Thursday, June 28, 2018 at 12:07 PM
To: Aaron Turkewitz <[log in to unmask]>
Subject: Re: Denis Lynn

I am very upset by this sad news. Year ago I met him in Prague at the Int. Congress of Protistology. It was so short time ago. Then He was interested in some commensalic ciliates (Chontricha) and next I tried, with not success, to find them on the Baltic shore. I remember Him as a person who was serving in many ways to the whole

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